Number of the records: 1
Affiblot: Semi-quantitative Selection and Characterization of Research Antibodies
- 1.
SYSNO ASEP 0545733 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Affiblot: Semi-quantitative Selection and Characterization of Research Antibodies Author(s) Svobodová, Z. (CZ)
Novotný, Jakub (UIACH-O) ORCID
Ospálková, B. (CZ)
Nemčeková, K. (CZ)
Tripská, K. (CZ)
Foret, František (UIACH-O) RID, ORCID
Bílková, Z. (CZ)Number of authors 7 Source Title MSB 2021 Final Program. -, 2021 Number of pages 1 s. Publication form Online - E Action International Symposium on Microscale Separations and Bioanalysis /37./ Event date 12.07.2021 - 15.07.2021 VEvent location Boston Country US - United States Event type WRD Language eng - English Country US - United States Keywords binding reagents ; rubella ; validation Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry Institutional support UIACH-O - RVO:68081715 Annotation The affiblot is a dot blot-based palm-sized device that researchers, laboratories, and institutions can use to semi-quantitatively evaluate and select the most suitable antibody among different suppliers that fit their application in terms of affinity comparison, cross-reactivity, and batch-to-batch variability. The device is also able to control the condition of the antibodies for the duration of their storage. Moreover, this simple, fast, and flexible technique does not require special equipment, except a vacuum pump and camera. If more precise results are required, the ChemiDoc imaging system can be used instead of the camera. The target can be a purified or recombinant peptide, protein, or whole bacterial cell. This device could be used to compare any antibodies or affinity reagents that have not yet been on the market for a long time and are therefore not yet thoroughly validated, such as anti-SARS-CoV2 antibodies. These antibodies are currently widely used to reveal the mechanisms of virus replication and host-to-host transmission, the development of diagnostic methods, and clinical research. The dot blot-based device could help researchers rapidly select the most suitable and functional antibody for their application. Therefore, our future intent will include the evaluation of several anti-SARS-CoV-2 antibodies for research use. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2022 Electronic address https://msb-conferences.org/detailed-program/
Number of the records: 1