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Direct evidence for crossover and chromatid interference in meiosis of two plant hybrids (Lolium multiflorumxFestuca pratensis and Allium cepaxA. roylei)
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SYSNO ASEP 0545209 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Direct evidence for crossover and chromatid interference in meiosis of two plant hybrids (Lolium multiflorumxFestuca pratensis and Allium cepaxA. roylei) Author(s) Ferreira, Marco Tulio Mendes (UEB-Q) ORCID
Glombik, Marek (UEB-Q) ORCID
Perničková, Kateřina (UEB-Q) ORCID
Duchoslav, M. (CZ)
Scholten, O. (NL)
Karafiátová, Miroslava (UEB-Q) RID, ORCID
Techio, V.H. (BR)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Lukaszewski, A.J. (US)
Kopecký, David (UEB-Q) RID, ORCIDNumber of authors 10 Source Title Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 72, č. 2 (2021), s. 254-267Number of pages 14 s. Language eng - English Country GB - United Kingdom Keywords crossing-over ; chiasma interference ; meiotic recombination ; tetrad analysis ; saccharomyces-cerevisiae ; individual chromosomes ; physical distribution ; statistical-analysis ; festuca-pratensis ; linked genes ; Centromere ; chromatid interference ; crossover interference ; homoeologous chromosome ; hybrid ; meiosis ; recombination OECD category Genetics and heredity (medical genetics to be 3) R&D Projects GA20-10019S GA ČR - Czech Science Foundation (CSF) EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000648886400010 EID SCOPUS 85102929034 DOI 10.1093/jxb/eraa455 Annotation Crossing over, in addition to its strictly genetic role, also performs a critical mechanical function, by bonding homologues in meiosis. Hence, it is responsible for an orderly reduction of the chromosome number. As such, it is strictly controlled in frequency and distribution. The well-known crossover control is positive crossover interference which reduces the probability of a crossover in the vicinity of an already formed crossover. A poorly studied aspect of the control is chromatid interference. Such analyses are possible in very few organisms as they require observation of all four products of a single meiosis. Here, we provide direct evidence of chromatid interference. Using in situ probing in two interspecific plant hybrids (Lolium multiflorumxFestuca pratensis and Allium cepaxA. roylei) during anaphase I, we demonstrate that the involvement of four chromatids in double crossovers is significantly more frequent than expected (64% versus 25%). We also provide a physical measure of the crossover interference distance, covering similar to 30-40% of the relative chromosome arm length, and show that the centromere acts as a barrier for crossover interference. The two arms of a chromosome appear to act as independent units in the process of crossing over. Chromatid interference has to be seriously addressed in genetic mapping approaches and further studies. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2022 Electronic address http://doi.org/10.1093/jxb/eraa455
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