Regioselective enzymatic hydrolysis of blackcurrant seed oil in supercritical CO2.

0544984 - ÚCHP 2022 RIV GB eng J - Journal Article
Pleskač, Ondřej - Sovová, Helena - Sajfrtová, Marie
Regioselective enzymatic hydrolysis of blackcurrant seed oil in supercritical CO2.
Journal of CO2 Utilization. Roč. 52, OCT 2021 (2021), č. článku 101692. ISSN 2212-9820. E-ISSN 2212-9839
R&D Projects: GA ČR(CZ) GA19-19245S
Institutional support: RVO:67985858
Keywords : hydrolysis * immobilized enzyme * vegetable oil
OECD category: Chemical engineering (plants, products)
Impact factor: 8.321, year: 2021
Method of publishing: Open access with time embargo

Continuous flow hydrolysis of blackcurrant seed oil dissolved in supercritical carbon dioxide (scCO2) was catalyzed by immobilized lipase Lipozyme TL IM. HPLC-MS and acid-base titration were used to determine composition of oil and hydrolysis products comprised of tri-, di- and monoglycerides, free fatty acids and glycerol. The oil contained 10 % linolenic and 2 % stearidonic fatty acids (omega-3), 51 % linoleic and 19 % gamma-linolenic fatty acids (omega-6), 12 % oleic acid, 5 % palmitic acid, and 1% stearic acid. Effects of enzyme load (17−500 mg), pressure (15−30 MPa), temperature (30−50 °C), and CO2 flow rate (0.2–4 g/min) on the conversion were evaluated. The reaction was reversible and the time to reach steady state was enzyme load-dependent and took up to 200 min. A slight drop of enzyme activity after four experimental runs and total reaction time 17 h was indicated by the decrease of free fatty acid content in the product from 0.72 % to 0.69 %. Due to the regioselectivity of lipase-catalyzed hydrolysis, the omega-3/omega-6 ratio was increased from 17:83 in oil to 23:77 in produced diglycerides.
Permanent Link: http://hdl.handle.net/11104/0321772