Expression and Localization of AβPP in SH-SY5Y Cells Depends on Differentiation State

0544239 - ÚFCH JH 2022 RIV NL eng J - Journal Article
Riegerová, Petra - Brejcha, Jindřich - Bezděková, D. - Chum, Tomáš - Mašínová, Eva - Čermáková, Nikola - Ovsepian, S. V. - Cebecauer, Marek - Štefl, Martin
Expression and Localization of AβPP in SH-SY5Y Cells Depends on Differentiation State.
Journal of Alzheimer's Disease. Roč. 82, č. 2 (2021), s. 485-491. ISSN 1387-2877. E-ISSN 1875-8908
R&D Projects: GA ČR(CZ) GJ19-08304Y
Grant - others:Akademie věd - GA AV ČR(CZ) L200401901
Institutional support: RVO:61388955
Keywords : AβPP * Alzheimer’s disease * differentiation
OECD category: Cell biology
Impact factor: 4.160, year: 2021
Method of publishing: Open access

Neuroblastoma cell line SH-SY5Y, due to its capacity to differentiate into neurons, easy handling, and low cost, is a common experimental model to study molecular events leading to Alzheimer's disease (AD). However, it is prevalently used in its undifferentiated state, which does not resemble neurons affected by the disease. Here, we show that the expression and localization of amyloid-β protein precursor (AβPP), one of the key molecules involved in AD pathogenesis, is dramatically altered in SH-SY5Y cells fully differentiated by combined treatment with retinoic acid and BDNF. We show that insufficient differentiation of SH-SY5Y cells results in AβPP mislocalization.

Permanent Link: http://hdl.handle.net/11104/0321269