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Therapeutic lithium alters polar head-group region of lipid bilayer and prevents lipid peroxidation in forebrain cortex of sleep-deprived rats
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SYSNO ASEP 0543857 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Therapeutic lithium alters polar head-group region of lipid bilayer and prevents lipid peroxidation in forebrain cortex of sleep-deprived rats Author(s) Vošahlíková, Miroslava (FGU-C) RID, ORCID, SAI
Roubalová, Lenka (FGU-C) RID, ORCID, SAI
Brejchová, Jana (FGU-C) RID, ORCID
Alda, M. (CZ)
Svoboda, Petr (FGU-C) RID, ORCIDArticle number 158962 Source Title Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. - : Elsevier - ISSN 1388-1981
Roč. 1866, č. 9 (2021)Number of pages 7 s. Language eng - English Country NL - Netherlands Keywords lithium ; rat forebrain cortex ; lipid bilayer ; membrane fluidity ; lipid peroxidation Subject RIV ED - Physiology OECD category Physiology (including cytology) R&D Projects GA17-07070S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support FGU-C - RVO:67985823 UT WOS 000665526800001 EID SCOPUS 85107019194 DOI https://doi.org/10.1016/j.bbalip.2021.158962 Annotation Lithium is regarded as a unique therapeutic agent for the management of bipolar disorder (BD). In efforts to explain the favourable effects of lithium in BD, a wide range of mechanisms was suggested. Among those, the effect of clinically relevant concentrations of lithium on the plasma membrane was extensively studied. However, the biophysical properties of brain membranes isolated from experimental animals exposed to acute, short-term and chronic lithium have not been performed to-date. In this study, we compared the biophysical parameters and level of lipid peroxidation in membranes isolated from forebrain cortex (FBC) of therapeutic lithium-treated and/ or sleep-deprived rats. Lithium interaction with FBC membranes was characterized by appropriate fluorescent probes. DPH (1,6-diphenyl-1,3,5-hexatriene) and TMA-DPH (1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene p-toluenesulphonate) were used for characterization of the hydrophobic lipid core and Laurdan (6-dodecanoyl-2-dimethylaminonaphthalene) for the membrane-water interface. Lipid peroxidation was determined by immunoblot analysis of 4-HNE-(4-hydroxynonenal)-protein adducts. The organization of polar headgroup region of FBC membranes, measured by Laurdan generalized polarization, was substantially altered by sleep deprivation and augmented by lithium treatment. Hydrophobic membrane interior characterized by steady-state anisotropy of DPH and TMA-DPH fluorescence was unchanged. Chronic lithium had a protective effect against peroxidative damage of membrane lipids in FBC. In summary, lithium administration at a therapeutic level and/or sleep deprivation as an animal model of mania resulted in changes in rat FBC membrane properties. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2022 Electronic address https://doi.org/10.1016/j.bbalip.2021.158962
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