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The Avian Retroviral Receptor Tva Mediates the Uptake of Transcobalamin Bound Vitamin B-12 (Cobalamin)
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SYSNO ASEP 0543825 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The Avian Retroviral Receptor Tva Mediates the Uptake of Transcobalamin Bound Vitamin B-12 (Cobalamin) Author(s) Krchlíková, Veronika (UMG-J)
Mikešová, J. (CZ)
Geryk, Josef (UMG-J) RID
Bařinka, Cyril (BTO-N) RID, ORCID
Nexo, E. (DK)
Fedosov, S.N. (DK)
Kosla, Jan (UMG-J) RID
Kučerová, Dana (UMG-J)
Reinišová, Markéta (UMG-J)
Hejnar, Jiří (UMG-J) RID
Elleder, Daniel (UMG-J) RIDNumber of authors 11 Article number e02136-20 Source Title Journal of Virology - ISSN 0022-538X
Roč. 95, č. 8 (2021)Number of pages 14 s. Publication form Online - E Language eng - English Country US - United States Keywords avian leukosis virus ; cobalamin ; receptor ; retrovirus Subject RIV EB - Genetics ; Molecular Biology OECD category Virology Subject RIV - cooperation Institute of Biotechnology - Microbiology, Virology R&D Projects LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA17-23675S GA ČR - Czech Science Foundation (CSF) LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support UMG-J - RVO:68378050 ; BTO-N - RVO:86652036 ; UMG-J - RVO:68378050 UT WOS 000636236300006 DOI 10.1128/JVI.02136-20 Annotation The avian sarcoma and leukosis viruses (ASLVs) are important chicken pathogens. Some of the virus subgroups, including ASLV-A and K, utilize the Tva receptor for cell entrance. Though Tva was identified 3 decades ago, its physiological function remains unknown. Previously, we have noted an intriguing resemblance and orthology between the chicken gene coding for Tva and the human gene coding for CD320, a receptor involved in cellular uptake of transcobalamin (TC) in complex with vitamin B12/cobalamin (Cbl). Here, we show that both the transmembrane and the glycosylphosphatidylinositol (GPI)-anchored form of Tva in the chicken cell line DF-1 promotes the uptake of Cbl with the help of expressed and purified chicken TC. The uptake of TC-Cbl complex was monitored using an isotope-or fluorophore-labeled Cbl. We show that (i) TC-Cbl is internalized in chicken cells, and (ii) the uptake is lower in the Tva-knockout cells and higher in Tva-overexpressing cells when compared with that of wild-type chicken cells. The relation between physiological function of Tva and its role in infection was elaborated by showing that infection with ASLV subgroups (targeting Tva) impairs the uptake of TC-Cbl, while this is not the case for cells infected with ASLV-B (not recognized by Tva). In addition, exposure of the cells to a high concentration of TC-Cbl alleviates the infection with Tva-dependent ASLV. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2022 Electronic address https://journals-asm-org.d360prx.biomed.cas.cz/doi/epub/10.1128/JVI.02136-20
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