Number of the records: 1
A Label-Free Diamond Microfluidic DNA Sensor Based on Active Nitrogen-Vacancy Center Charge State Control
- 1.
SYSNO ASEP 0542602 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A Label-Free Diamond Microfluidic DNA Sensor Based on Active Nitrogen-Vacancy Center Charge State Control Tvůrce(i) Krečmarová, M. (CZ)
Gulka, Michal (UOCHB-X) ORCID
Vandenryt, T. (BE)
Hrubý, J. (BE)
Fekete, Ladislav (FZU-D) RID, ORCID
Hubík, Pavel (FZU-D) RID, ORCID
Taylor, Andrew (FZU-D) RID, ORCID
Mortet, Vincent (FZU-D) RID, ORCID
Thoelen, R. (BE)
Bourgeois, E. (BE)
Nesládek, M. (CZ)Zdroj.dok. ACS Applied Materials and Interfaces. - : American Chemical Society - ISSN 1944-8244
Roč. 13, č. 16 (2021), s. 18500-18510Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova nitrogen-vacancy center ; diamond ; biosensor ; microfluidic ; DNA chip Obor OECD Biophysics Vědní obor RIV – spolupráce Fyzikální ústav - Biotechnologie a bionika CEP GA16-16336S GA ČR - Grantová agentura ČR LM2015088 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LO1409 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Fellowship J. E. Purkyně GA AV ČR - Akademie věd Způsob publikování Omezený přístup Institucionální podpora UOCHB-X - RVO:61388963 ; FZU-D - RVO:68378271 UT WOS 000645520700010 EID SCOPUS 85105095597 DOI https://doi.org/10.1021/acsami.1c01118 Anotace We propose a label-free biosensor concept based on the charge state manipulation of nitrogen-vacancy (NV) quantum color centers in diamond, combined with an electrochemical microfluidic flow cell sensor, constructed on boron-doped diamond. This device can be set at a defined electrochemical potential, locking onto the particular chemical reaction, whilst the NV center provides the sensing function. The NV charge state occupation is initially prepared by applying a bias voltage on a gate electrode and then subsequently altered by exposure to detected charged molecules. We demonstrate the functionality of the device by performing label-free optical detection of DNA molecules. In this experiment, a monolayer of strongly cationic charged polymer polyethylenimine is used to shift the charge state of near surface NV centers from negatively charged NV– to neutral NV0 or dark positively charged NV+. Immobilization of negatively charged DNA molecules on the surface of the sensor restores the NV centers charge state back to the negatively charged NV–, which is detected using confocal photoluminescence microscopy. Biochemical reactions in the microfluidic channel are characterized by electrochemical impedance spectroscopy. The use of the developed electrochemical device can also be extended to nuclear magnetic resonance spin sensing. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2022 Elektronická adresa https://doi.org/10.1021/acsami.1c01118
Number of the records: 1