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The Gut Microbiota Affects Corticosterone Production in the Murine Small Intestine
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SYSNO ASEP 0542413 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The Gut Microbiota Affects Corticosterone Production in the Murine Small Intestine Author(s) Ergang, Peter (FGU-C) RID, ORCID
Vagnerová, Karla (FGU-C) RID, ORCID
Hermanová, Petra (MBU-M) ORCID
Vodička, Martin (FGU-C) RID, ORCID, SAI
Jágr, M. (CZ)
Šrůtková, Dagmar (MBU-M) ORCID, RID
Dvořáček, V. (CZ)
Hudcovic, Tomáš (MBU-M) RID, ORCID
Pácha, Jiří (FGU-C) RID, ORCIDArticle number 4229 Source Title International Journal of Molecular Sciences. - : MDPI - ISSN 1661-6596
Roč. 22, č. 8 (2021)Number of pages 15 s. Language eng - English Country CH - Switzerland Keywords glucocorticoids ; extra-adrenal steroidogenesis ; 11beta-hydroxysteroid dehydrogenase ; intestine ; microbiome ; anti-CD3 antibody Subject RIV ED - Physiology OECD category Physiology (including cytology) Subject RIV - cooperation Institute of Microbiology - Biochemistry R&D Projects GA18-02993S GA ČR - Czech Science Foundation (CSF) GA21-10845S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support FGU-C - RVO:67985823 ; MBU-M - RVO:61388971 UT WOS 000644332500001 EID SCOPUS 85104404619 DOI https://doi.org/10.3390/ijms22084229 Annotation Glucocorticoids (GCs) are hormones that are released in response to stressors and exhibit many activities, including immunomodulatory and anti-inflammatory activities. They are primarily synthesized in the adrenal gland but are also produced in peripheral tissues via regeneration of adrenal 11-oxo metabolites or by de novo synthesis from cholesterol. The present study investigated the influence of the microbiota on de novo steroidogenesis and regeneration of corticosterone in the intestine of germ-free (GF) and specific pathogen-free mice challenged with a physical stressor (anti-CD3 antibody i.p. injection). In the small intestine, acute immune stress resulted in increased mRNA levels of the proinflammatory cytokines IL1 beta, IL6 and Tnf alpha and genes involved in de novo steroidogenesis (Stard3 and Cyp11a1), as well as in regeneration of active GCs from their 11-oxo metabolites (Hsd11b1). GF mice showed a generally reduced transcriptional response to immune stress, which was accompanied by decreased intestinal corticosterone production and reduced expression of the GC-sensitive marker Fkbp5. In contrast, the interaction between stress and the microbiota was not detected at the level of plasma corticosterone or the transcriptional response of adrenal steroidogenic enzymes. The results indicate a differential immune stress-induced intestinal response to proinflammatory stimuli and local corticosterone production driven by the gut microbiota. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2022 Electronic address https://www.mdpi.com/1422-0067/22/8/4229
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