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Hypersensitivity Induced by Intrathecal Bradykinin Administration Is Enhanced by N-oleoyldopamine (OLDA) and Prevented by TRPV1 Antagonist
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SYSNO ASEP 0542140 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Hypersensitivity Induced by Intrathecal Bradykinin Administration Is Enhanced by N-oleoyldopamine (OLDA) and Prevented by TRPV1 Antagonist Author(s) Uchytilová, Eva (FGU-C)
Špicarová, Diana (FGU-C) RID, ORCID
Paleček, Jiří (FGU-C) RID, ORCIDArticle number 3712 Source Title International Journal of Molecular Sciences. - : MDPI - ISSN 1661-6596
Roč. 22, č. 7 (2021)Number of pages 12 s. Language eng - English Country CH - Switzerland Keywords TRPV1 ; bradykinin ; OLDA ; spinal cord ; hyperalgesia ; allodynia Subject RIV FH - Neurology OECD category Neurosciences (including psychophysiology R&D Projects GA20-19136S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 000638655100001 EID SCOPUS 85103479134 DOI https://doi.org/10.3390/ijms22073712 Annotation Transient receptor potential vanilloid 1 (TRPV1) channels contribute to the development of several chronic pain states and represent a possible therapeutic target in many painful disease treatment. Proinflammatory mediator bradykinin (BK) sensitizes TRPV1, whereas noxious peripheral stimulation increases BK level in the spinal cord. Here, we investigated the involvement of spinal TRPV1 in thermal and mechanical hypersensitivity, evoked by intrathecal (i.t.) administration of BK and an endogenous agonist of TRPV1, N-oleoyldopamine (OLDA), using behavioral tests and i.t. catheter implantation, and administration of BK-induced transient thermal and mechanical hyperalgesia and mechanical allodynia. All these hypersensitive states were enhanced by co-administration of a low dose of OLDA (0.42 mu g i.t.), which was ineffective only under the control conditions. Intrathecal pretreatment with TRPV1 selective antagonist SB366791 prevented hypersensitivity induced by i.t. co-administration of BK and OLDA. Our results demonstrate that both thermal and mechanical hypersensitivity evoked by co-administration of BK and OLDA is mediated by the activation of spinal TRPV1 channels. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2022 Electronic address https://www.mdpi.com/1422-0067/22/7/3712
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