Number of the records: 1  

The intestinal microbiota and metabolites in patients with anorexia nervosa

  1. 1.
    SYSNO ASEP0542104
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleThe intestinal microbiota and metabolites in patients with anorexia nervosa
    Author(s) Procházková, Petra (MBU-M) RID, ORCID
    Roubalová, Radka (MBU-M) RID, ORCID
    Dvořák, Jiří (MBU-M) RID, ORCID
    Kreisinger, J. (CZ)
    Hill, M. (CZ)
    Tlaskalová-Hogenová, Helena (MBU-M) RID, ORCID
    Tomášová, Petra (MBU-M)
    Pelantová, Helena (MBU-M) ORCID, RID
    Čermáková, Martina (MBU-M) ORCID
    Kuzma, Marek (MBU-M) ORCID, RID
    Bulant, J. (CZ)
    Bilej, Martin (MBU-M) RID, ORCID
    Smitka, K. (CZ)
    Lambertová, A. (CZ)
    Holanová, P. (CZ)
    Papežová, H. (CZ)
    Article numbere1902771
    Source TitleGut Microbes. - : Taylor & Francis - ISSN 1949-0976
    Roč. 13, č. 1 (2021)
    Number of pages26 s.
    Languageeng - English
    CountryUS - United States
    KeywordsMicrobiome ; bacteriome ; mycobiome ; scfa ; neurotransmitter ; ede-q ; bmi ; dysbiosis ; renourishment ; gut-brain-microbiota axis
    Subject RIVEE - Microbiology, Virology
    OECD categoryMicrobiology
    R&D ProjectsNV17-28905A GA MZd - Ministry of Health (MZ)
    NV18-01-00040 GA MZd - Ministry of Health (MZ)
    Method of publishingOpen access
    Institutional supportMBU-M - RVO:61388971
    UT WOS000634902300001
    EID SCOPUS85103370703
    DOI https://doi.org/10.1080/19490976.2021.1902771
    AnnotationBrain-gut microbiota interactions are intensively studied in connection with various neurological and psychiatric diseases. While anorexia nervosa (AN) pathophysiology is not entirely clear, it is presumably linked to microbiome dysbiosis. We aimed to elucidate the gut microbiota contribution in AN disease pathophysiology. We analyzed the composition and diversity of the gut microbiome of patients with AN (bacteriome and mycobiome) from stool samples before and after renourishment, and compared them to healthy controls. Further, levels of assorted neurotransmitters and short-chain fatty acids (SCFA) were analyzed in stool samples by MS and NMR, respectively. Biochemical, anthropometric, and psychometric profiles were assessed. The bacterial alpha-diversity parameter analyses revealed only increased Chao 1 index in patients with AN before the realimentation, reflecting their interindividual variation. Subsequently, core microbiota depletion signs were observed in patients with AN. Overrepresented OTUs (operation taxonomic units) in patients with AN taxonomically belonged to Alistipes, Clostridiales, Christensenellaceae, and Ruminococcaceae. Underrepresented OTUs in patients with AN were Faecalibacterium, Agathobacter, Bacteroides, Blautia, and Lachnospira. Patients exhibited greater interindividual variation in the gut bacteriome, as well as in metagenome content compared to controls, suggesting altered bacteriome functions. Patients had decreased levels of serotonin, GABA, dopamine, butyrate, and acetate in their stool samples compared to controls. Mycobiome analysis did not reveal significant differences in alpha diversity and fungal profile composition between patients with AN and healthy controls, nor any correlation of the fungal composition with the bacterial profile. Our results show the changed profile of the gut microbiome and its metabolites in patients with severe AN.
    WorkplaceInstitute of Microbiology
    ContactEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Year of Publishing2022
    Electronic addresshttps://www.tandfonline.com/doi/full/10.1080/19490976.2021.1902771
Number of the records: 1  

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