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The intestinal microbiota and metabolites in patients with anorexia nervosa
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SYSNO ASEP 0542104 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The intestinal microbiota and metabolites in patients with anorexia nervosa Author(s) Procházková, Petra (MBU-M) RID, ORCID
Roubalová, Radka (MBU-M) RID, ORCID
Dvořák, Jiří (MBU-M) RID, ORCID
Kreisinger, J. (CZ)
Hill, M. (CZ)
Tlaskalová-Hogenová, Helena (MBU-M) RID, ORCID
Tomášová, Petra (MBU-M)
Pelantová, Helena (MBU-M) ORCID, RID
Čermáková, Martina (MBU-M) ORCID
Kuzma, Marek (MBU-M) ORCID, RID
Bulant, J. (CZ)
Bilej, Martin (MBU-M) RID, ORCID
Smitka, K. (CZ)
Lambertová, A. (CZ)
Holanová, P. (CZ)
Papežová, H. (CZ)Article number e1902771 Source Title Gut Microbes. - : Taylor & Francis - ISSN 1949-0976
Roč. 13, č. 1 (2021)Number of pages 26 s. Language eng - English Country US - United States Keywords Microbiome ; bacteriome ; mycobiome ; scfa ; neurotransmitter ; ede-q ; bmi ; dysbiosis ; renourishment ; gut-brain-microbiota axis Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects NV17-28905A GA MZd - Ministry of Health (MZ) NV18-01-00040 GA MZd - Ministry of Health (MZ) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000634902300001 EID SCOPUS 85103370703 DOI https://doi.org/10.1080/19490976.2021.1902771 Annotation Brain-gut microbiota interactions are intensively studied in connection with various neurological and psychiatric diseases. While anorexia nervosa (AN) pathophysiology is not entirely clear, it is presumably linked to microbiome dysbiosis. We aimed to elucidate the gut microbiota contribution in AN disease pathophysiology. We analyzed the composition and diversity of the gut microbiome of patients with AN (bacteriome and mycobiome) from stool samples before and after renourishment, and compared them to healthy controls. Further, levels of assorted neurotransmitters and short-chain fatty acids (SCFA) were analyzed in stool samples by MS and NMR, respectively. Biochemical, anthropometric, and psychometric profiles were assessed. The bacterial alpha-diversity parameter analyses revealed only increased Chao 1 index in patients with AN before the realimentation, reflecting their interindividual variation. Subsequently, core microbiota depletion signs were observed in patients with AN. Overrepresented OTUs (operation taxonomic units) in patients with AN taxonomically belonged to Alistipes, Clostridiales, Christensenellaceae, and Ruminococcaceae. Underrepresented OTUs in patients with AN were Faecalibacterium, Agathobacter, Bacteroides, Blautia, and Lachnospira. Patients exhibited greater interindividual variation in the gut bacteriome, as well as in metagenome content compared to controls, suggesting altered bacteriome functions. Patients had decreased levels of serotonin, GABA, dopamine, butyrate, and acetate in their stool samples compared to controls. Mycobiome analysis did not reveal significant differences in alpha diversity and fungal profile composition between patients with AN and healthy controls, nor any correlation of the fungal composition with the bacterial profile. Our results show the changed profile of the gut microbiome and its metabolites in patients with severe AN. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://www.tandfonline.com/doi/full/10.1080/19490976.2021.1902771
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