Structure of SRSF1 RRM1 bound to RNA reveals an unexpected bimodal mode of interaction and explains its involvement in SMN1 exon7 splicing

0541951 - BFÚ 2022 RIV GB eng J - Journal Article
Clery, A. - Krepl, Miroslav - Nguyen, C. K. X. - Moursy, A. - Jorjani, H. - Katsantoni, M. - Okoniewski, M. - Mittal, N. - Zavolan, M. - Šponer, Jiří - Allain, F.H.T.
Structure of SRSF1 RRM1 bound to RNA reveals an unexpected bimodal mode of interaction and explains its involvement in SMN1 exon7 splicing.
Nature Communications. Roč. 12, č. 1 (2021), č. článku 428. E-ISSN 2041-1723
R&D Projects: GA ČR(CZ) GA20-16554S
Institutional support: RVO:68081707
Keywords : pre-messenger-rna * spinal muscular-atrophy * protein-protein interactions * nmr structure determination * sr protein * recognition motifs
OECD category: Physical chemistry
Impact factor: 17.694, year: 2021
Method of publishing: Open access
https://www.nature.com/articles/s41467-020-20481-w

The human prototypical SR protein SRSF1 is an oncoprotein that contains two RRMs and plays a pivotal role in RNA metabolism. We determined the structure of the RRM1 bound to RNA and found that the domain binds preferentially to a CN motif (N is for any nucleotide). Based on this solution structure, we engineered a protein containing a single glutamate to asparagine mutation (E87N), which gains the ability to bind to uridines and thereby activates SMN exon7 inclusion, a strategy that is used to cure spinal muscular atrophy. Finally, we revealed that the flexible inter-RRM linker of SRSF1 allows RRM1 to bind RNA on both sides of RRM2 binding site. Besides revealing an unexpected bimodal mode of interaction of SRSF1 with RNA, which will be of interest to design new therapeutic strategies, this study brings a new perspective on the mode of action of SRSF1 in cells.
Permanent Link: http://hdl.handle.net/11104/0319465