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Bactericidal effect of zinc oxide nanoparticles on Gram-positive and Gram-negative strains in reverse spin bioreactor

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    0541850 - FZÚ 2022 RIV GB eng C - Conference Paper (international conference)
    Rutherford, D. - Jíra, J. - Kolářová, Kateřina - Remeš, Zdeněk - Rezek, B.
    Bactericidal effect of zinc oxide nanoparticles on Gram-positive and Gram-negative strains in reverse spin bioreactor.
    IOP Conference Series: Materials Science and Engineering. Vol. 1050. Bristol: IOP Publishing Ltd, 2021 - (Kozisek, Z.; Behulova, Z.; Potucek, Z.), č. článku 012013. ISSN 1757-8981.
    [Joint Seminar on Development of Materials Science in Research and Education /30./ DMSRE 2020. Pavlov (CZ), 07.09.2020-11.09.2020]
    R&D Projects: GA MŠMT(CZ) EF16_019/0000760; GA ČR GC19-02858J; GA MŠMT LM2018110
    Grant - others:OP VVV - SOLID21(XE) CZ.02.1.01/0.0/0.0/16_019/0000760
    Institutional support: RVO:68378271
    Keywords : ZnO * reverse spin bioreactor
    OECD category: Biophysics

    We present an experimental study investigating the efficacy of zinc oxide nanoparticle use for bacterial decontamination of water using reverse spin technology. The number of viable bacteria reduced with treatment time and the rate of inactivation was dependent on zinc oxide concentration. Gram-positive S. aureus bacteria were more resistant to zinc oxide nanoparticles compared to Gram-negative E. coli, however both strains were completely eradicated after 4 hours using a concentration of 10 μg/mL. Nanoparticles did not inhibit growth of bacteria when added to an agar surface, neither when in liquid before bacteria inoculation nor when loaded onto filter paper disks after bacteria inoculation. These results emphasise the importance of efficient reactor mixing to enhance interaction capability for ZnO use in water decontamination.
    Permanent Link: http://hdl.handle.net/11104/0319360

     
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