Growth Factors VEGF-A(165) and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation

Sedlář, Antonín; Trávníčková, Martina; Matějka, Roman; Pražák, Šimon; Mészáros, Zuzana; Bojarová, Pavla; Bačáková, Lucie; Křen, Vladimír; Slámová, Kristýna

doi:https://dx.doi.org/10.3390/ijms22041843

Number of the records: 1

Growth Factors VEGF-A(165) and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation

1.

SYSNO ASEP	0541641
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Growth Factors VEGF-A(165) and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation
Author(s)	Sedlář, Antonín (FGU-C)_ORCID Trávníčková, Martina (FGU-C)_{RID, ORCID, SAI} Matějka, Roman (FGU-C)_{RID, ORCID, SAI} Pražák, Šimon (FGU-C)_ORCID Mészáros, Zuzana (MBU-M) Bojarová, Pavla (MBU-M)_ORCID Bačáková, Lucie (FGU-C)_{RID, ORCID} Křen, Vladimír (MBU-M)_{RID, ORCID} Slámová, Kristýna (MBU-M)_{RID, ORCID}
Article number	1843
Source Title	International Journal of Molecular Sciences. - : MDPI Roč. 22, č. 4 (2021)
Number of pages	27 s.
Language	eng - English
Country	CH - Switzerland
Keywords	heterologous expression ; recombinant vascular endothelial growth factor (VEGF) ; basic fibroblast growth factor (bFGF) ; adult stem cells ; endothelial cells ; cell adhesion ; cell proliferation ; tissue engineering ; regenerative medicine ; vascular replacements
Subject RIV	EI - Biotechnology ; Bionics
OECD category	Biomaterials (as related to medical implants, devices, sensors)
Subject RIV - cooperation	Institute of Microbiology - Biochemistry
R&D Projects	GA18-01163S GA ČR - Czech Science Foundation (CSF)
	NV18-02-00422 GA MZd - Ministry of Health (MZ)
	LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LTC18038 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	FGU-C - RVO:67985823 ; MBU-M - RVO:61388971
UT WOS	000623786800001
EID SCOPUS	85100662467
DOI	10.3390/ijms22041843
Annotation	Vascular endothelial growth factor-A(165) (VEGF-A(165)) and fibroblast growth factor-2 (FGF-2) are currently used for the functionalization of biomaterials designed for tissue engineering. We have developed a new simple method for heterologous expression and purification of VEGF-A(165) and FGF-2 in the yeast expression system of Pichia pastoris. The biological activity of the growth factors was assessed in cultures of human and porcine adipose tissue-derived stem cells (ADSCs) and human umbilical vein endothelial cells (HUVECs). When added into the culture medium, VEGF-A(165) stimulated proliferation only in HUVECs, while FGF-2 stimulated the proliferation of both cell types. A similar effect was achieved when the growth factors were pre-adsorbed to polystyrene wells. The effect of our recombinant growth factors was slightly lower than that of commercially available factors, which was attributed to the presence of some impurities. The stimulatory effect of the VEGF-A(165) on cell adhesion was rather weak, especially in ADSCs. FGF-2 was a potent stimulator of the adhesion of ADSCs but had no to negative effect on the adhesion of HUVECs. In sum, FGF-2 and VEGF-A(165) have diverse effects on the behavior of different cell types, which maybe utilized in tissue engineering.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2022
Electronic address	https://www.mdpi.com/1422-0067/22/4/1843

Number of the records: 1