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Missing Information from the Estrogen Receptor Puzzle: Where Are They Localized in Bull Reproductive Tissues and Spermatozoa?

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    0541326 - BTÚ 2021 RIV CH eng J - Journal Article
    Antalíková, J. - Šecová, P. - Horovská, L. - Krejčířová, R. - Šimoník, Ondřej - Jankovičová, J. - Bartokova, M. - Tůmová, L. - Maňásková-Postlerová, Pavla
    Missing Information from the Estrogen Receptor Puzzle: Where Are They Localized in Bull Reproductive Tissues and Spermatozoa?
    Cells. Roč. 9, č. 1 (2020), č. článku 183. E-ISSN 2073-4409
    R&D Projects: GA MŠMT(CZ) ED1.1.00/02.0109
    Institutional support: RVO:86652036
    Keywords : reproduction * epididymis * steroid hormones * bovine
    OECD category: Cell biology
    Impact factor: 6.600, year: 2020
    Method of publishing: Open access
    https://www.mdpi.com/2073-4409/9/1/183

    Estrogens are steroid hormones that affect a wide range of physiological functions. The effect of estrogens on male reproductive tissues and sperm cells through specific receptors is essential for sperm development, maturation, and function. Although estrogen receptors (ERs) have been studied in several mammalian species, including humans, they have not yet been described in bull spermatozoa and reproductive tissues. In this study, we analyzed the presence of all types of ERs (ESR1, ESR2, and GPER1) in bull testicular and epididymal tissues and epididymal and ejaculated spermatozoa, and we characterize them here for the first time. We observed different localizations of each type of ER in the sperm head by immunofluorescent microscopy. Additionally, using a selected polyclonal antibody, we found that each type of ER in bull sperm extracts had two isoforms with different molecular masses. The detailed detection of ERs is a prerequisite not only for understanding the effect of estrogen on all reproductive events but also for further studying the negative effect of environmental estrogens (endocrine disruptors) on processes that lead to fertilization.
    Permanent Link: http://hdl.handle.net/11104/0318897

     
     
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