Number of the records: 1
The Rho guanine nucleotide exchange factor Trio is required for neural crest cell migration and interacts with Dishevelled
- 1.0540503 - BFÚ 2021 RIV GB eng J - Journal Article
Kratzer, M.-C. - Becker, S. F. S. - Grund, A. - Merks, A. - Harnoš, J. - Bryja, Vítězslav - Giehl, K. - Kashef, J. - Borchers, A.
The Rho guanine nucleotide exchange factor Trio is required for neural crest cell migration and interacts with Dishevelled.
Development. Roč. 147, č. 10 (2020), č. článku dev186338. ISSN 0950-1991. E-ISSN 1477-9129
Institutional support: RVO:68081707
Keywords : intellectual disability * signaling pathways * beta-catenin * gef domain * xenopus * rac
OECD category: Developmental biology
Impact factor: 6.868, year: 2020
Method of publishing: Open access
https://dev.biologists.org/content/147/10/dev186338
Directional migration during embryogenesis and tumor progression faces the challenge that numerous external signals need to converge to precisely control cell movement. The Rho guanine exchange factor (GEF) Trio is especially well suited to relay signals, as it features distinct catalytic domains to activate Rho GTPases. Here, we show that Trio is required for Xenopus cranial neural crest (NC) cell migration and cartilage formation. Trio cell-autonomously controls protrusion formation of NC cells and Trio morphant NC cells show a blebbing phenotype. Interestingly, the Trio GEF2 domain is sufficient to rescue protrusion formation and migration of Trio morphant NC cells. We show that this domain interacts with the DEP/C-terminus of Dishevelled (DVL). DVL but not a deletion construct lacking the DEP domain is able to rescue protrusion formation and migration of Trio morphant NC cells. This is likely mediated by activation of Reel, as we find that DVL rescues Rac1 activity in Trio morphant embryos. Thus, our data provide evidence for a novel signaling pathway, whereby Trio controls protrusion formation of cranial NC cells by interacting with DVL to activate Rac1.
Permanent Link: http://hdl.handle.net/11104/0318124
Number of the records: 1