Key Mechanistic Principles and Considerations Concerning RNA Interference

0539799 - ÚMG 2021 RIV CH eng J - Journal Article
Svoboda, Petr
Key Mechanistic Principles and Considerations Concerning RNA Interference.
Frontiers in Plant Science. Roč. 11, August (2020), č. článku 1237. ISSN 1664-462X. E-ISSN 1664-462X
R&D Projects: GA MŠMT LO1419
Institutional support: RVO:68378050
Keywords : RNAi * dicer * argonaute * miRNA * dsRNA * off-targeting
OECD category: Biochemistry and molecular biology
Impact factor: 5.754, year: 2020
Method of publishing: Open access
https://www.frontiersin.org/articles/10.3389/fpls.2020.01237/full

Canonical RNAi, one of the so-called RNA-silencing mechanisms, is defined as sequence-specific RNA degradation induced by long double-stranded RNA (dsRNA). RNAi occurs in four basic steps: (i) processing of long dsRNA by RNase III Dicer into small interfering RNA (siRNA) duplexes, (ii) loading of one of the siRNA strands on an Argonaute protein possessing endonucleolytic activity, (iii) target recognition through siRNA basepairing, and (iv) cleavage of the target by the Argonaute's endonucleolytic activity. This basic pathway diversified and blended with other RNA silencing pathways employing small RNAs. In some organisms, RNAi is extended by an amplification loop employing an RNA-dependent RNA polymerase, which generates secondary siRNAs from targets of primary siRNAs. Given the high specificity of RNAi and its presence in invertebrates, it offers an opportunity for highly selective pest control. The aim of this text is to provide an introductory overview of key mechanistic aspects of RNA interference for understanding its potential and constraints for its use in pest control.
Permanent Link: http://hdl.handle.net/11104/0317500