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Expression quantitative trait loci in ABC transporters are associated with survival in 5-FU treated colorectal cancer patients

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    SYSNO ASEP0539307
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleExpression quantitative trait loci in ABC transporters are associated with survival in 5-FU treated colorectal cancer patients
    Author(s) Vymetálková, Veronika (UEM-P) RID
    Rosa, F. (IT)
    Susová, S. (CZ)
    Bendová, Petra (UEM-P)
    Levý, M. (CZ)
    Buchler, T. (CZ)
    Král, J. (CZ)
    Bártů, Linda (UEM-P)
    Vodičková, Ludmila (UEM-P) RID
    Hughes, D.J. (DE)
    Souček, P. (CZ)
    Naccarati, Alessio (UEM-P)
    Kumar, R. (DE)
    Vodička, Pavel (UEM-P) RID
    Pardini, B. (IT)
    Source TitleMutagenesis. - : Oxford University Press - ISSN 0267-8357
    Roč. 35, č. 3 (2020), s. 273-281
    Number of pages9 s.
    Languageeng - English
    CountryGB - United Kingdom
    Keywordsclass-i antigen ; down-regulation ; gene-expression
    Subject RIVFR - Pharmacology ; Medidal Chemistry
    OECD categoryPharmacology and pharmacy
    R&D ProjectsNV19-09-00237 GA MZd - Ministry of Health (MZ)
    GA17-16857S GA ČR - Czech Science Foundation (CSF)
    Method of publishingLimited access
    Institutional supportUEM-P - RVO:68378041
    UT WOS000593117600006
    EID SCOPUS85088201245
    DOI https://doi.org/10.1093/mutage/gez050
    AnnotationThe chemotherapeutic efficacy in colorectal cancer (CRC) is limited due to the inter-individual variability in drug response and the development of tumour resistance. ATP-binding cassette (ABC) transporters are crucial in the development of resistance by the efflux of anticancer agents from cancer cells. In this study, we identified 14 single nucleotide polymorphisms (SNPs) in 11 ABC transporter genes acting as an expression of quantitative trait loci (eQTLs), i.e. whose variation influence the expression of many downstream genes. These SNPs were genotyped in a case-control study comprising 1098 cases and 1442 healthy controls and analysed in relation to CRC development risk and patient survival. Considering a strict correction for multiple tests, we did not observe any significant association between SNPs and CRC risk. The rs3819720 polymorphism in the ABCB3/TAP2 gene was statistically significantly associated with shorter overall survival (OS) in the codominant, and dominant models [GA vs. GG, hazard ratio (HR) = 1.48, P= 0.002, AA vs. GG, HR = 1.70, P= 0.004 and GA + AA vs. GG, HR = 1.52, P= 0.0006]. Additionally, GA carriers of the same SNP displayed worse OS after receiving 5-FU based chemotherapy. The variant allele of rs3819720 polymorphism statistically significantly affected the expression of 36 downstream genes. Screening for eQTL polymorphisms in relevant genes such as ABC transporters that can regulate the expression of several other genes may help to identify the genetic background involved in the individual response to the treatment of CRC patients.
    WorkplaceInstitute of Experimental Medicine
    ContactArzuv Čaryjeva, arzuv.caryjeva@iem.cas.cz, Tel.: 241 062 218, 296 442 218
    Year of Publishing2021
    Electronic addresshttps://academic.oup.com/mutage/article-abstract/35/3/273/5699895?redirectedFrom=fulltext
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