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Proteomic Analysis of Whole Saliva in Relation to Dental Caries Resistance
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SYSNO ASEP 0538460 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Proteomic Analysis of Whole Saliva in Relation to Dental Caries Resistance Author(s) Kulhavá, Lucie (FGU-C) ORCID
Eckhardt, Adam (FGU-C) RID, ORCID
Pataridis, Statis (FGU-C)
Foltán, R. (CZ)
Mikšík, Ivan (FGU-C) RID, ORCID, SAISource Title Folia Biologica. - : Univerzita Karlova v Praze - ISSN 0015-5500
Roč. 66, č. 2 (2020), s. 72-80Number of pages 9 s. Language eng - English Country CZ - Czech Republic Keywords dental caries ; fractions of whole human saliva ; label-free quantification ; mass spectrometry ; proteins Subject RIV EI - Biotechnology ; Bionics OECD category Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions (pharmacogenomics, gene-based therapeutics) R&D Projects NV17-31564A GA MZd - Ministry of Health (MZ) Method of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 000573917500004 EID SCOPUS 85089978631 Annotation Saliva contains possible biomarkers that are associated with dental caries. The present study aimed to analyse differences in the abundance of proteins in the saliva between caries-positive (CP, N = 15) and caries-free (CF, N = 12) males and to compare differences in the abundance of proteins between two saliva sample fractions (supernatant and pellet). We found 14 differently significantly expressed proteins in the CF group when comparing the supernatant fractions of the CP and CF groups, and three proteins in the pellet fractions had significantly higher expression in the CP group. Our results indicate very specific protein compositions of the saliva in relation to dental caries resistance (the saliva of the CP group mainly contained pellet proteins and the saliva of the CF group mainly contained supernatant proteins). This was the first time that the saliva pellet fraction was analysed in relation to the dental caries status. We detected specific calcium-binding proteins that could have decalcified enamel in the saliva pellet of the CP group. We also observed significantly up-regulated immune proteins in the saliva supernatant of the CF group that could play an important role in the caries prevention. The particular protein compositions of the saliva pellet and supernatant in the groups with different susceptibilities to tooth decay is a promising finding for future research. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2021 Electronic address https://fb.cuni.cz/file/5923/fb2020a0009.pdf
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