Functionalized hydrogel microparticles prepared by microfluidics and their interaction with tumour marker carbonic anhydrase IX

0537848 - ÚMG 2021 RIV GB eng J - Journal Article
Pittermannová, A. - Ruberova, Z. - Lizonova, D. - Hubatova-Vackova, A. - Kaspar, O. - Zadrazil, A. - Král, Vlastimil - Pechar, Michal - Pola, Robert - Bibette, J. - Bremond, N. - Štěpánek, F. - Tokárová, V.
Functionalized hydrogel microparticles prepared by microfluidics and their interaction with tumour marker carbonic anhydrase IX.
Soft Matter. Roč. 16, č. 37 (2020), s. 8702-8709. ISSN 1744-683X. E-ISSN 1744-6848
Institutional support: RVO:68378050 ; RVO:61389013
Keywords : alginate microspheres * delivery * nanoparticles * release * microcapsules * encapsulation * fabrication * generation * liposomes * gel
OECD category: Biochemistry and molecular biology; Polymer science (UMCH-V)
Impact factor: 3.679, year: 2020
Method of publishing: Limited access
https://pubs.rsc.org/en/content/articlelanding/2020/SM/D0SM01018A#!divAbstract

Microfluidics allows precise control of the synthesis of microparticles for specific applications, where size and morphology play an important role. In this work, we have introduced microfluidic chip design with dedicated extraction and gelation sections allowing to prepare hydrogel particles in the size range of a red blood cell. The influence of the extractive channel size, alginate concentration and type of storage media on the final size of the prepared alginate microparticles has been discussed. The second part of the work is dedicated to the surface modification of prepared particles using chitosan, pHPMA and the monoclonal antibody molecule, IgG M75. The specific interaction of the antibody molecule with an antigen domain of carbonic anhydrase IX, the transmembrane tumour protein associated with several types of cancer, is demonstrated by fluorescence imaging and compared to an isotypic antibody molecule.
Permanent Link: http://hdl.handle.net/11104/0315789