0537796 - BFÚ 2021 RIV US eng J - Journal Article
Vašíček, Ondřej - Hájek, Jan - Blahová, L. - Hrouzek, Pavel - Babica, Pavel - Kubala, Lukáš - Šindlerová, Lenka
Cyanobacterial lipopeptides puwainaphycins and minutissamides induce disruptive and pro-inflammatory processes in Caco-2 human intestinal barrier model.
Harmful Algae. Roč. 96, Jun 2020 (2020), č. článku 101849. ISSN 1568-9883. E-ISSN 1878-1470
R&D Projects: GA ČR(CZ) GA16-24949S; GA MŠMT(CZ) LO1416
Institutional support: RVO:68081707 ; RVO:61388971 ; RVO:67985939
Keywords : cell * peptides * proliferation * stabilization * toxicity * claudins * health
OECD category: Microbiology; Marine biology, freshwater biology, limnology (MBU-M); Toxicology (BU-J)
Impact factor: 4.273, year: 2020 ; AIS: 0.994, rok: 2020
Method of publishing: Limited access
Result website:
https://doi.org/10.1016/j.hal.2020.101849DOI: https://doi.org/10.1016/j.hal.2020.101849

Puwainaphycins (PUW) and minutissamides (MIN) are cyanobacterial lipopeptides found in various cyanobacterial species. The first possible target of human exposure to them is intestinal epithelium but effect of PUW/ MIN on enterocytes is not known at all. Using differentiated Caco-2 cells, PUW F was found to be cytotoxic from 5 mu M concentration based on lactate dehydrogenase release assay and total protein concentration. However, it is also able to induce production of interleukin 8 in non-cytotoxic concentrations 1 and 2.5 mu M detected by ELISA. Effects of MIN A and C were similar but less pronounced compared to PUW F. On the other hand, MIN D was the least toxic compound with no significant pro-inflammatory effects. Surprisingly, pro-inflammatory activation of the cells by PUW F and MIN C resulted in an increase in tight junction (TJ) protein claudin 4 expression determined by western blot analysis and confirmed by confocal microscopy. Furthermore, decrease in expression of zonula occludens 3, another TJ protein, was observed after the exposure to PUW F. Taken together, these cytotoxic lipopeptides, especially PUW F, are to be studied more deeply due to their capability to activate and/or deregulate human enterocytes in low concentrations.
Permanent Link: http://hdl.handle.net/11104/0315638