Number of the records: 1
Resolving the taxonomy of emerging zoonotic pathogens in the Trichophyton benhamiae complex
- 1.
SYSNO ASEP 0536753 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Resolving the taxonomy of emerging zoonotic pathogens in the Trichophyton benhamiae complex Author(s) Čmoková, Adéla (MBU-M) ORCID
Kolařík, Miroslav (MBU-M) RID, ORCID
Dobiáš, R. (CZ)
Hoyer, L. L. (US)
Janouškovcová, H. (CZ)
Kano, R. (JP)
Kuklová, I. (CZ)
Lysková, P. (CZ)
Machová, Lenka (MBU-M)
Maier, T. (DE)
Mallátová, N. (CZ)
Man, Matěj (BU-J) ORCID
Mencl, K. (CZ)
Nenoff, P. (DE)
Peano, A. (IT)
Prausová, H. (CZ)
Stubbe, D. (BE)
Uhrlass, S. (DE)
Větrovský, Tomáš (MBU-M) ORCID, RID
Wiegand, C. (DE)
Hubka, Vít (MBU-M) ORCIDSource Title Fungal Diversity. - : Springer - ISSN 1560-2745
Roč. 104, č. 1 (2020), s. 333-387Number of pages 55 s. Language eng - English Country US - United States Keywords Epizootic fungal infections ; Microsatellite typing scheme ; Multigene phylogeny ; Population genetic structure ; Superficial skin infections ; Zoophilic dermatophytes Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects NV17-31269A GA MZd - Ministry of Health (MZ) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support MBU-M - RVO:61388971 ; BU-J - RVO:67985939 UT WOS 000592613100001 EID SCOPUS 85096540372 DOI 10.1007/s13225-020-00465-3 Annotation Species of the Trichophyton benhamiae complex are predominantly zoophilic pathogens with a worldwide distribution. These pathogens have recently become important due to their epidemic spread in pets and pet owners. Considerable genetic and phenotypic variability has been revealed in these emerging pathogens, but the species limits and host spectra have not been clearly elucidated. In this study, we used an approach combining phylogenetic analysis based on four loci, population-genetic data, phenotypic and physiological analysis, mating type gene characterization and ecological data to resolve the taxonomy of these pathogens. This approach supported the inclusion of nine taxa in the complex, including three new species and one new variety. Trichophyton benhamiae var. luteum var. nov. (´yellow phenotype´ strains) is currently a major cause of zoonotic tinea corporis and capitis in Europe (mostly transmitted from guinea pigs). The isolates of the ´white phenotype´ do not form a monophyletic group and are segregated into three taxa, T. benhamiae var. benhamiae (mostly North America, dogs), T. europaeum sp. nov. (mostly Europe, guinea pigs), and T. japonicum sp. nov. (predominant in East Asia but also found in Europe, rabbits and guinea pigs). The new species T. africanum sp. nov. is proposed for the ´African´ race of T. benhamiae. The introduction to new geographic areas and host jump followed by extinction of one mating type gene have played important roles in the evolution of these pathogens. Due to considerable phenotypic similarity of many dermatophytes and phenomena such as incomplete lineage sorting or occasional hybridization and introgression, we demonstrate the need to follow polyphasic approach in species delimitation. Neutrally evolving and noncoding DNA regions showed significantly higher discriminatory power compared to conventional protein-coding loci. Diagnostic options for species identification in practice based on molecular markers, phenotype and MALDI-TOF spectra are presented. A microsatellite typing scheme developed in this study is a powerful tool for the epidemiological surveillance of these emerging pathogens. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2021 Electronic address https://link.springer.com/article/10.1007%2Fs13225-020-00465-3
Number of the records: 1