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Coherent anti-Stokes Raman scattering microscopy through a multimode fiber endoscope
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SYSNO ASEP 0535569 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Coherent anti-Stokes Raman scattering microscopy through a multimode fiber endoscope Author(s) Traegaardh, Johanna (UPT-D) RID, ORCID, SAI
Pikálek, Tomáš (UPT-D) RID, ORCID, SAI
Šerý, Mojmír (UPT-D) RID, SAI
Akimov, D. (DE)
Meyer, T. (DE)
Poppe, J. (DE)
Čižmár, Tomáš (UPT-D) RID, ORCID, SAINumber of authors 7 Article number 162267 Source Title Biomedical Spectroscopy, Microscopy, and Imaging 2020. Proceedings of SPIE, 11359. - Bellingham : SPIE, 2020 - ISSN 0277-786X - ISBN 978-151063490-9 Number of pages 9 s. Publication form Online - E Action Biomedical Spectroscopy, Microscopy, and Imaging 2020 Event date 06.04.2020 - 10.04.2020 VEvent location France Country FR - France Event type WRD Language eng - English Country US - United States Keywords adaptive optics ; CARS ; endoscopy ; microscopy ; multi-photon microscopy ; Raman Subject RIV BH - Optics, Masers, Lasers OECD category Optics (including laser optics and quantum optics) R&D Projects EF15_003/0000476 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UPT-D - RVO:68081731 UT WOS 000674749600004 EID SCOPUS 85090399464 DOI 10.1117/12.2555080 Annotation Advanced wavefront-shaping methods can be used to transform a simple multimode fiber into an ultra-thin laser scanning microscope. Here, we extend this technique to label-free non-linear microscopy with chemical contrast using coherent anti-Stokes Raman scattering (CARS) through a multimode fiber endoscope, which opens up new avenues for instant and in-situ diagnosis of potentially malignant tissue. We use a commercial, 125 μm diameter, 0.29 NA, GRIN fiber as the endoscopic probe. Wavefront shaping on a spatial light modulator is used to create a focus, where the 1-2 ps long pump and Stokes pulses are overlapped in time, which is scanned behind the fiber facet across the sample. The chemical selectivity is demonstrated by imaging 2 μm polystyrene and 2.5 μm PMMA beads with per pixel integration time as low as 1 ms for epi-detection. Epi-detection through the fiber is possible despite the fact that the CARS signal is emitted mainly in the forward direction, away from the fiber facet. Detecting the back-scattered signal from the underlying tissue, requires a large detector aperture to be efficient. By detecting through both the core and the cladding of the fiber, we obtain sufficient detection efficiency. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2021 Electronic address https://www.spiedigitallibrary.org/conference-proceedings-of-spie/11359/1135907/Coherent-anti-Stokes-Raman-scattering-microscopy-through-a-multimode-fiber/10.1117/12.2555080.short
Number of the records: 1