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Polarized Sonic Hedgehog Protein Localization and a Shift in the Expression of Region-Specific Molecules Is Associated With the Secondary Palate Development in the Veiled Chameleon
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SYSNO ASEP 0532001 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Polarized Sonic Hedgehog Protein Localization and a Shift in the Expression of Region-Specific Molecules Is Associated With the Secondary Palate Development in the Veiled Chameleon Author(s) Hampl, M. (CZ)
Dumková, J. (CZ)
Kavková, M. (CZ)
Dosedělová, H. (CZ)
Bryjová, A. (CZ)
Zahradníček, Oldřich (UJF-V) ORCID, SAI
Pyszko, M. (CZ)
Macholán, M. (CZ)
Zikmund, T. (CZ)
Kaiser, J. (CZ)
Buchtová, M. (CZ)Number of authors 11 Article number 572 Source Title Frontiers in Cell and Developmental Biology. - : Frontiers Research Foundation - ISSN 2296-634X
Roč. 8, JUL (2020)Number of pages 29 s. Publication form Print - P Language eng - English Country CH - Switzerland Keywords secondary palate ; SHH ; primary cilia ; skeletogenesis ; chamaleon ; reptile Subject RIV BO - Biophysics OECD category Biophysics Method of publishing Open access Institutional support UJF-V - RVO:61389005 UT WOS 000561204000001 EID SCOPUS 85089346331 DOI 10.3389/fcell.2020.00572 Annotation Secondary palate development is characterized by the formation of two palatal shelves on the maxillary prominences, which fuse in the midline in mammalian embryos. However, in reptilian species, such as turtles, crocodilians, and lizards, the palatal shelves of the secondary palate develop to a variable extent and morphology. While in most Squamates, the palate is widely open, crocodilians develop a fully closed secondary palate. Here, we analyzed developmental processes that underlie secondary palate formation in chameleons, where large palatal shelves extend horizontally toward the midline. The growth of the palatal shelves continued during post-hatching stages and closure of the secondary palate can be observed in several adult animals. The massive proliferation of a multilayered oral epithelium and mesenchymal cells in the dorsal part of the palatal shelves underlined the initiation of their horizontal outgrowth, and was decreased later in development. The polarized cellular localization of primary cilia and Sonic hedgehog protein was associated with horizontal growth of the palatal shelves. Moreover, the development of large palatal shelves, supported by the pterygoid and palatine bones, was coupled with the shift inMeox2,Msx1, andPax9gene expression along the rostro-caudal axis. In conclusion, our results revealed distinctive developmental processes that contribute to the expansion and closure of the secondary palate in chameleons and highlighted divergences in palate formation across amniote species. Workplace Nuclear Physics Institute Contact Markéta Sommerová, sommerova@ujf.cas.cz, Tel.: 266 173 228 Year of Publishing 2021 Electronic address https://doi.org/10.3389/fcell.2020.00572
Number of the records: 1