Number of the records: 1
Jasmonate-independent regulation of digestive enzyme activity in the carnivorous butterwort Pinguicula x Tina
- 1.
SYSNO ASEP 0531938 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Jasmonate-independent regulation of digestive enzyme activity in the carnivorous butterwort Pinguicula x Tina Author(s) Kocáb, O. (CZ)
Jakšová, J. (CZ)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Petřík, Ivan (UEB-Q) ORCID
Lenobel, R. (CZ)
Chamrád, I. (CZ)
Pavlovič, A. (CZ)Number of authors 7 Source Title Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 71, č. 12 (2020), s. 3749-3758Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords proteome analysis ; abscisic-acid ; pitcher fluid ; prey capture ; glands ; lentibulariaceae ; proteins ; plants ; genus ; ultrastructure ; Butterwort ; carnivorous plant ; digestive enzymes ; electrical signals ; jasmonic acid ; Pinguicula ; protease ; variation potential Subject RIV EF - Botanics OECD category Biochemistry and molecular biology R&D Projects EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000553121200034 EID SCOPUS 85090265590 DOI 10.1093/jxb/eraa159 Annotation Carnivorous plants within the order Caryophyllales use jasmonates, a class of phytohormone, in the regulation of digestive enzyme activities. We used the carnivorous butterwort Pinguicula x Tina from the order Lamiales to investigate whether jasmonate signaling is a universal and ubiquitous signaling pathway that exists outside the order Caryophyllales. We measured the electrical signals, enzyme activities, and phytohormone tissue levels in response to prey capture. Mass spectrometry was used to identify proteins in the digestive secretion. We identified eight enzymes in the digestive secretion, many of which were previously found in other genera of carnivorous plants. Among them, alpha-amylase is unique in carnivorous plants. Enzymatic activities increased in response to prey capture, however, the tissue content of jasmonic acid and its isoleucine conjugate remained rather low in contrast to the jasmonate response to wounding. Enzyme activities did not increase in response to the exogenous application of jasmonic acid or coronatine. Whereas similar digestive enzymes were co-opted from plant defense mechanisms among carnivorous plants, the mode of their regulation differs. The butterwort has not co-opted jasmonate signaling for the induction of enzyme activities in response to prey capture. Moreover, the presence of alpha-amylase in digestive fluid of P. x Tina, which has not been found in other genera of carnivorous plants, might indicate that non-defense-related genes have also been co-opted for carnivory. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2021 Electronic address http://doi.org/10.1093/jxb/eraa159
Number of the records: 1