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Multi-component antioxidative system and robust carbohydrate status, the essence of plant arsenic tolerance
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SYSNO ASEP 0531599 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Multi-component antioxidative system and robust carbohydrate status, the essence of plant arsenic tolerance Author(s) Kofroňová, M. (CZ)
Hrdinová, A. (CZ)
Mašková, P. (CZ)
Tremlová, J. (CZ)
Soudek, Petr (UEB-Q) RID, ORCID
Petrová, Šárka (UEB-Q) RID, ORCID
Pinkas, D. (CZ)
Lipavská, H. (CZ)Number of authors 8 Article number 283 Source Title Antioxidants. - : MDPI
Roč. 9, č. 4 (2020)Number of pages 21 s. Language eng - English Country CH - Switzerland Keywords Antioxidant ; Antioxidant enzyme ; Arsenate ; Arsenic ; Arsenite ; Oxidative stress ; ros ; Saccharides ; Tolerant and sensitive tobacco Subject RIV EA - Cell Biology OECD category Cell biology Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000536961200033 EID SCOPUS 85084655162 DOI 10.3390/antiox9040283 Annotation Arsenic (As) contaminates the food chain and decreases agricultural production through impairing plants, particularly due to oxidative stress. To better understand the As tolerance mechanisms, two contrasting tobacco genotypes: As-sensitive Nicotiana sylvestris and As-tolerant N.tabacum, cv. ‘Wisconsin’ were analyzed. The most meaningful differences were found in the carbohydrate status, neglected so far in the As context. In the tolerant genotype, contrary to the sensitive one, net photosynthesis rates and saccharide levels were unaffected by As exposure. Importantly, the total antioxidant capacity was far stronger in the As-tolerant genotype, based on higher antioxidants levels (e.g., phenolics, ascorbate, glutathione) and activities and/or appropriate localizations of antioxidative enzymes, manifested as reverse root/shoot activities in the selected genotypes. Accordingly, malondialdehyde levels, a lipid peroxidation marker, increased only in sensitive tobacco, indicating efficient membrane protection in As-tolerant species. We bring new evidence of the orchestrated action of a broad spectrum of both antioxidant enzymes and molecules essential for As stress coping. For the first time, we propose robust carbohydrate metabolism based on undisturbed photosynthesis to be crucial not only for subsidizing C and energy for defense but also for participating in direct reactive oxygen species (ROS) quenching. The collected data and suggestions can serve as a basis for the selection of plant As phytoremediators or for targeted breeding of tolerant crops. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2021 Electronic address http://doi.org/10.3390/antiox9040283
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