A Novel Ruthenium Based Coordination Compound Against Pathogenic Bacteria

0525046 - ÚVGZ 2021 RIV CH eng J - Journal Article
Sur, V. P. - Mazumdar, A. - Kopel, P. - Mukherjee, S. - Vítek, Petr - Michalkova, H. - Vaculovičová, M. - Moulick, A.
A Novel Ruthenium Based Coordination Compound Against Pathogenic Bacteria.
International Journal of Molecular Sciences. Roč. 21, č. 7 (2020), č. článku 2656. E-ISSN 1422-0067
Research Infrastructure: CzeCOS III - 90123
Institutional support: RVO:86652079
Keywords : staphylococcus-aureus * antimicrobial agent * quantum dots * schiff-bases * complexes * antibacterial * nanoparticles * methicillin * model * metalloproteinases * coordination compound * antimicrobial compound * ruthenium * benzimidazole * sem * eds
OECD category: Biophysics
Impact factor: 5.924, year: 2020
Method of publishing: Open access
https://www.mdpi.com/1422-0067/21/7/2656

The current epidemic of antibiotic-resistant infections urges to develop alternatives to less-effective antibiotics. To assess anti-bacterial potential, a novel coordinate compound (RU-S4) was synthesized using ruthenium-Schiff base-benzimidazole ligand, where ruthenium chloride was used as the central atom. RU-S4 was characterized by scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDS), and Raman spectroscopy. Antibacterial effect of RU-S4 was studied against Staphylococcus aureus (NCTC 8511), vancomycin-resistant Staphylococcus aureus (VRSA) (CCM 1767), methicillin-resistant Staphylococcus aureus (MRSA) (ST239: SCCmecIIIA), and hospital isolate Staphylococcus epidermidis. The antibacterial activity of RU-S4 was checked by growth curve analysis and the outcome was supported by optical microscopy imaging and fluorescence LIVE/DEAD cell imaging. In vivo (balb/c mice) infection model prepared with VRSA (CCM 1767) and treated with RU-S4. In our experimental conditions, all infected mice were cured. The interaction of coordination compound with bacterial cells were further confirmed by cryo-scanning electron microscope (Cryo-SEM). RU-S4 was completely non-toxic against mammalian cells and in mice and subsequently treated with synthesized RU-S4.
Permanent Link: http://hdl.handle.net/11104/0309261