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Division of Labor Between Two Actin Nucleators—the Formin FH1 and the ARP2/3 Complex—in Arabidopsis Epidermal Cell Morphogenesis
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SYSNO ASEP 0524770 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Division of Labor Between Two Actin Nucleators—the Formin FH1 and the ARP2/3 Complex—in Arabidopsis Epidermal Cell Morphogenesis Author(s) Cifrová, P. (CZ)
Oulehlová, Denisa (UEB-Q) RID
Kollárová, E. (CZ)
Martinek, J. (CZ)
Rosero, A. (CZ)
Žárský, Viktor (UEB-Q) RID, ORCID
Schwarzerová, K. (CZ)
Cvrčková, F. (CZ)Number of authors 8 Article number 148 Source Title Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 11, MAR 2 (2020)Number of pages 17 s. Language eng - English Country CH - Switzerland Keywords actin nucleation ; arp2/3 ; At3g25500 ; At4g01710 ; cytoskeleton ; formin ; pavement cell ; trichome Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemical research methods R&D Projects EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000524735000001 EID SCOPUS 85082724884 DOI 10.3389/fpls.2020.00148 Annotation The ARP2/3 complex and formins are the only known plant actin nucleators. Besides their actin-related functions, both systems also modulate microtubule organization and dynamics. Loss of the main housekeeping Arabidopsis thaliana Class I membrane-targeted formin FH1 (At3g25500) is known to increase cotyledon pavement cell lobing, while mutations affecting ARP2/3 subunits exhibit an opposite effect. Here we examine the role of FH1 and the ARP2/3 complex subunit ARPC5 (At4g01710) in epidermal cell morphogenesis with focus on pavement cells and trichomes using a model system of single fh1 and arpc5, as well as double fh1 arpc5 mutants. While cotyledon pavement cell shape in double mutants mostly resembled single arpc5 mutants, analysis of true leaf epidermal morphology, as well as actin and microtubule organization and dynamics, revealed a more complex relationship between the two systems and similar, rather than antagonistic, effects on some parameters. Both fh1 and arpc5 mutations increased actin network density and increased cell shape complexity in pavement cells and trichomes of first true leaves, in contrast to cotyledons. Thus, while the two actin nucleation systems have complementary roles in some aspects of cell morphogenesis in cotyledon pavement cells, they may act in parallel in other cell types and developmental stages. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2021 Electronic address http://doi.org/10.3389/fpls.2020.00148
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