Doxorubicin‐conjugated iron oxide nanoparticles: surface engineering and biomedical investigation

0524743 - ÚMCH 2021 RIV DE eng J - Journal Article
Oleksa, Viktoriia - Macková, Hana - Patsula, Vitalii - Dydowiczová, Aneta - Janoušková, Olga - Horák, Daniel
Doxorubicin‐conjugated iron oxide nanoparticles: surface engineering and biomedical investigation.
ChemPlusChem. Roč. 85, č. 6 (2020), s. 1156-1163. ISSN 2192-6506. E-ISSN 2192-6506
R&D Projects: GA ČR(CZ) GC20-02177J
Institutional support: RVO:61389013
Keywords : antitumor agents * cytotoxicity * drug delivery
OECD category: Polymer science
Impact factor: 2.863, year: 2020
Method of publishing: Limited access
https://chemistry-europe.onlinelibrary.wiley.com/doi/full/10.1002/cplu.202000360

Development of therapeutic systems to treat glioblastoma, the most common and aggressive brain tumor, belongs to priority tasks in cancer research. We have synthesized colloidally stable magnetic nanoparticles (D h=336 nm) coated with doxorubicin (Dox) conjugated copolymers of N,N ‐dimethylacrylamide and either N ‐acryloylglycine methyl ester or N ‐acryloylmethyl 6‐aminohexanoate. The terminal carboxyl groups of the copolymers were reacted with alendronate by carbodiimide formation. Methyl ester groups were then transferred to hydrazides for binding Dox by a hydrolytically labile hydrazone bond. The polymers were subsequently bound on the magnetic nanoparticles through bisphosphonate terminal groups. Finally, the anticancer effect of the Dox‐conjugated particles was investigated using the U‐87 glioblastoma cell line in terms of particle internalization and cell viability, which decreased to almost zero at a concentration of 100 μg of particles per ml. These results confirmed that poly(N,N ‐dimethylacrylamide)‐coated magnetic nanoparticles can serve as a solid support for Dox delivery to glioblastoma cells.
Permanent Link: http://hdl.handle.net/11104/0309384