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Mmi1, the Yeast Ortholog of Mammalian Translationally Controlled Tumor Protein (TCTP), Negatively Affects Rapamycin-Induced Autophagy in Post-Diauxic Growth Phase
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SYSNO ASEP 0524512 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Mmi1, the Yeast Ortholog of Mammalian Translationally Controlled Tumor Protein (TCTP), Negatively Affects Rapamycin-Induced Autophagy in Post-Diauxic Growth Phase Author(s) Vojtová, Jana (MBU-M) ORCID
Hašek, Jiří (MBU-M) RID, ORCIDArticle number 138 Source Title Cells. - : MDPI
Roč. 9, č. 1 (2020)Number of pages 17 s. Language eng - English Country CH - Switzerland Keywords Mmi1 ; TCTP ; translationally controlled tumor protein Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA16-05497S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000515398200138 EID SCOPUS 85088464285 DOI 10.3390/cells9010138 Annotation Translationally controlled tumor protein (TCTP) is a multifunctional and highly conserved protein from yeast to humans. Recently, its role in non-selective autophagy has been reported with controversial results in mammalian and human cells. Herein we examine the effect of Mmi1, the yeast ortholog of TCTP, on non-selective autophagy in budding yeast Saccharomyces cerevisiae, a well-established model system to monitor autophagy. We induced autophagy by nitrogen starvation or rapamycin addition and measured autophagy by using the Pho8 Delta 60 and GFP-Atg8 processing assays in WT, mmi1 Delta, and in autophagy-deficient strains atg8 Delta or atg1 Delta. Our results demonstrate that Mmi1 does not affect basal or nitrogen starvation-induced autophagy. However, an increased rapamycin-induced autophagy is detected in mmi1 Delta strain when the cells enter the post-diauxic growth phase, and this phenotype can be rescued by inserted wild-type MMI1 gene. Further, the mmi1 Delta cells exhibit significantly lower amounts of reactive oxygen species (ROS) in the post-diauxic growth phase compared to WT cells. In summary, our study suggests that Mmi1 negatively affects rapamycin-induced autophagy in the post-diauxic growth phase and supports the role of Mmi1/TCTP as a negative autophagy regulator in eukaryotic cells. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2021 Electronic address https://www.mdpi.com/2073-4409/9/1/138
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