CRMP2 mediates Sema3F‐dependent axon pruning and dendritic spine remodeling

Žiak, Jakub; Weissová, Romana; Jeřábková, Kateřina; Janíková, Martina; Maimon, R.; Petrásek, Tomáš; Pukajová, Barbora; Kleisnerová, Marie; Wang, M.; Brill, M. S.; Kašpárek, Petr; Zhou, X.; Alvarez-Bolado, G.; Sedláček, Radislav; Misgeld, T.; Stuchlík, Aleš; Perlson, E.; Balaštík, Martin

doi:https://dx.doi.org/10.15252/embr.201948512

Number of the records: 1

CRMP2 mediates Sema3F‐dependent axon pruning and dendritic spine remodeling

1.

SYSNO ASEP	0524173
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	CRMP2 mediates Sema3F‐dependent axon pruning and dendritic spine remodeling
Author(s)	Žiak, Jakub (FGU-C)_{RID, ORCID} Weissová, Romana (FGU-C)_{ORCID, RID, SAI} Jeřábková, Kateřina (UMG-J) Janíková, Martina (FGU-C)_ORCID Maimon, R. (IL) Petrásek, Tomáš (FGU-C)_{RID, ORCID, SAI} Pukajová, Barbora (FGU-C)_{ORCID, RID} Kleisnerová, Marie (FGU-C)_{ORCID, RID} Wang, M. (DE) Brill, M. S. (DE) Kašpárek, Petr (UMG-J) Zhou, X. (DE) Alvarez-Bolado, G. (DE) Sedláček, Radislav (UMG-J)_RID Misgeld, T. (DE) Stuchlík, Aleš (FGU-C)_{RID, ORCID} Perlson, E. (IL) Balaštík, Martin (FGU-C)_{RID, ORCID}
Article number	e48512
Source Title	Embo Reports - ISSN 1469-221X Roč. 21, č. 3 (2020)
Number of pages	22 s.
Language	eng - English
Country	US - United States
Keywords	axon guidance ; collapsin response mediator protein 2 ; dendritic spines ; semaphorins ; synapse pruning
Subject RIV	FH - Neurology
OECD category	Neurosciences (including psychophysiology
R&D Projects	NV18-04-00085 GA MZd - Ministry of Health (MZ)
	GA16-15915S GA ČR - Czech Science Foundation (CSF)
	GA19-03016S GA ČR - Czech Science Foundation (CSF)
	NV17-30833A GA MZd - Ministry of Health (MZ)
	LM2015040 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED2.1.00/19.0395 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LO1419 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	FGU-C - RVO:67985823 ; UMG-J - RVO:68378050
UT WOS	000506383200001
EID SCOPUS	85078597836
DOI	10.15252/embr.201948512
Annotation	Regulation of axon guidance and pruning of inappropriate synapses by class 3 semaphorins are key to the development of neural circuits. Collapsin response mediator protein 2 (CRMP2) has been shown to regulate axon guidance by mediating semaphorin 3A (Sema3A) signaling, however, nothing is known about its role in synapse pruning. Here, using newly generated crmp2−/− mice we demonstrate that CRMP2 has a moderate effect on Sema3A‐dependent axon guidance in vivo, and its deficiency leads to a mild defect in axon guidance in peripheral nerves and the corpus callosum. Surprisingly, crmp2−/− mice display prominent defects in stereotyped axon pruning in hippocampus and visual cortex and altered dendritic spine remodeling, which is consistent with impaired Sema3F signaling and with models of autism spectrum disorder (ASD). We demonstrate that CRMP2 mediates Sema3F signaling in primary neurons and that crmp2−/− mice display ASD‐related social behavior changes in the early postnatal period as well as in adults. Together, we demonstrate that CRMP2 mediates Sema3F‐dependent synapse pruning and its dysfunction shares histological and behavioral features of ASD.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2021
Electronic address	https://doi.org/10.15252/embr.201948512

Number of the records: 1