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Advances in Disease Models
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SYSNO ASEP 0523240 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Exploring avian cancer genome with insertional mutagenesis screens using myeloblastosis associated viruses Author(s) Pečenka, Vladimír (UMG-J)
Pajer, Petr (UMG-J)
Karafiát, Vít (UMG-J)
Dvořák, Michal (UMG-J) RIDSource Title Advances in Disease Models. - Praha : OPTIO CZ, 2019 / Bartůněk Petr - ISBN 978-80-88011-06-4 Pages s. 189-206 Number of pages 18 s. Number of copy 50 Number of pages 310 Publication form Print - P Language eng - English Country CZ - Czech Republic Keywords chicken retrovirus myeloblastosis-associated virus-2 ; insertional mutagenesis ; oncogenesis Subject RIV EB - Genetics ; Molecular Biology OECD category Virology R&D Projects LO1419 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UMG-J - RVO:68378050 Annotation We have established a new model for studying cancer genetics based on insertional mutagenesis by chicken retrovirus myeloblastosis-associated virus-2 (MAV-2) and tumor-promoting agents that target tumorigenesis into individual organs. Using high-performance iPCR we analyzed a large number of chicken tumors, pinpointed common provirus-insertion sites and associated them with candidate cancer genes. So far, we have defined the principal oncogenes driving formation of chicken nephroblastomas (FOXP1, PLAG1, TWIST or HRAS), lung hemangiosarcomas (FRK), liver hemangiosarcomas (HRAS), hepatocarcinomas (EGFR), and cholangiocarcinomas (HGFR or MST1R)(1-4). All of them are now proven human oncogenes, in three cases (TWIST, FOXP1, FRK), our findings preceded the discovery of their involvement in human tumors. This shows that non-mouse species are good alternative models for studying oncogenesis with outcomes applicable to human medicine.
Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2020
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