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Colloidally stable polypeptide‐based nanogel: study of enzyme‐mediated nanogelation in inverse miniemulsion

  1. 1.
    SYSNO ASEP0522680
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleColloidally stable polypeptide‐based nanogel: study of enzyme‐mediated nanogelation in inverse miniemulsion
    Author(s) Dvořáková, Jana (UMCH-V) RID, ORCID
    Šálek, Petr (UMCH-V) RID, ORCID
    Korecká, L. (CZ)
    Pavlova, Ewa (UMCH-V) RID
    Černoch, Peter (UMCH-V) RID, ORCID
    Janoušková, Olga (UMCH-V) RID, SAI, ORCID
    Koutníková, Barbora (UMCH-V)
    Proks, Vladimír (UMCH-V) RID, ORCID
    Article number48725
    Source TitleJournal of Applied Polymer Science. - : Wiley - ISSN 0021-8995
    Roč. 137, č. 21 (2020), s. 1-10
    Number of pages10 s.
    Languageeng - English
    CountryUS - United States
    Keywordsbiocompatible ; biodegradable ; human plasma
    Subject RIVEI - Biotechnology ; Bionics
    OECD categoryBioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
    R&D ProjectsGA18-03224S GA ČR - Czech Science Foundation (CSF)
    Method of publishingLimited access
    Institutional supportUMCH-V - RVO:61389013
    UT WOS000496555800001
    EID SCOPUS85075144046
    DOI10.1002/app.48725
    AnnotationThe current work presents a pivotal study of the nanogelation of the linear poly(N5‐2‐hydroxypropyl‐L‐glutamine) polymer precursor containing tyramine (TYR) units in an inverse miniemulsion by horseradish peroxidase/H2O2‐mediated crosslinking. The effects of various nH2O2/nTYR ratios on the kinetics of nanogelation in the inverse miniemulsion and on the reaction time are investigated by linear sweep voltammetry, while the formation of dityramine crosslinking is explored by fluorescence spectroscopy. The study is completed using dynamic light scattering measurements, nanoparticle tracking analysis, and cryogenic transmission electron microscopy to acquire comprehensive information about the formed nanoparticulate systems. With the optimal ratio nH2O2/nTYR = 2, the strategy yields in the high‐quality ~ 130 nm poly(amino acid)‐based nanogel, which is prepared in 2 h. The nanogel is colloidally stable under different temperature and pH conditions for over 168 h. Moreover, the demonstrated nanogel is noncytotoxic for HeLa cells and human primary fibroblasts and is quickly enzymatically hydrolyzed into small fragments during a biodegradation study in human blood plasma.
    WorkplaceInstitute of Macromolecular Chemistry
    ContactEva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
    Year of Publishing2021
    Electronic addresshttps://onlinelibrary.wiley.com/doi/full/10.1002/app.48725
Number of the records: 1  

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