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Colloidally stable polypeptide‐based nanogel: study of enzyme‐mediated nanogelation in inverse miniemulsion
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SYSNO ASEP 0522680 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Colloidally stable polypeptide‐based nanogel: study of enzyme‐mediated nanogelation in inverse miniemulsion Author(s) Dvořáková, Jana (UMCH-V) RID, ORCID
Šálek, Petr (UMCH-V) RID, ORCID
Korecká, L. (CZ)
Pavlova, Ewa (UMCH-V) RID
Černoch, Peter (UMCH-V) RID, ORCID
Janoušková, Olga (UMCH-V) RID, SAI, ORCID
Koutníková, Barbora (UMCH-V)
Proks, Vladimír (UMCH-V) RID, ORCIDArticle number 48725 Source Title Journal of Applied Polymer Science. - : Wiley - ISSN 0021-8995
Roč. 137, č. 21 (2020), s. 1-10Number of pages 10 s. Language eng - English Country US - United States Keywords biocompatible ; biodegradable ; human plasma Subject RIV EI - Biotechnology ; Bionics OECD category Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials R&D Projects GA18-03224S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support UMCH-V - RVO:61389013 UT WOS 000496555800001 EID SCOPUS 85075144046 DOI 10.1002/app.48725 Annotation The current work presents a pivotal study of the nanogelation of the linear poly(N5‐2‐hydroxypropyl‐L‐glutamine) polymer precursor containing tyramine (TYR) units in an inverse miniemulsion by horseradish peroxidase/H2O2‐mediated crosslinking. The effects of various nH2O2/nTYR ratios on the kinetics of nanogelation in the inverse miniemulsion and on the reaction time are investigated by linear sweep voltammetry, while the formation of dityramine crosslinking is explored by fluorescence spectroscopy. The study is completed using dynamic light scattering measurements, nanoparticle tracking analysis, and cryogenic transmission electron microscopy to acquire comprehensive information about the formed nanoparticulate systems. With the optimal ratio nH2O2/nTYR = 2, the strategy yields in the high‐quality ~ 130 nm poly(amino acid)‐based nanogel, which is prepared in 2 h. The nanogel is colloidally stable under different temperature and pH conditions for over 168 h. Moreover, the demonstrated nanogel is noncytotoxic for HeLa cells and human primary fibroblasts and is quickly enzymatically hydrolyzed into small fragments during a biodegradation study in human blood plasma. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2021 Electronic address https://onlinelibrary.wiley.com/doi/full/10.1002/app.48725
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