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Restricted and Non-Essential Redundancy of RNAi and piRNA Pathways in Mouse Oocytes
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SYSNO ASEP 0521819 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Restricted and Non-Essential Redundancy of RNAi and piRNA Pathways in Mouse Oocytes Author(s) Táborská, Eliška (UMG-J)
Pasulka, Josef (UMG-J)
Malík, Radek (UMG-J) RID
Horvat, Filip (UMG-J)
Jeníčková, Irena (UMG-J)
Matoševič, Z.J. (HR)
Svoboda, Petr (UMG-J) RIDArticle number e1008261 Source Title PLoS Genetics. - : Public Library of Science - ISSN 1553-7404
Roč. 15, č. 12 (2019)Number of pages 22 s. Publication form Online - E Language eng - English Country US - United States Keywords DOUBLE-STRANDED-RNA ; GENE-EXPRESSION ; RETROTRANSPOSON SUBFAMILY ; MAMMALIAN OOCYTES ; PIWI PROTEINS MILI ; IDENTIFICATION ; AMPLIFICATION ; TRANSCRIPTION ; INTERFERENCE Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) R&D Projects LO1419 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015040 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED2.1.00/19.0395 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015040 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED2.1.00/19.0395 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UMG-J - RVO:68378050 UT WOS 000512336600004 DOI 10.1371/journal.pgen.1008261 Annotation Germline genome defense evolves to recognize and suppress retrotransposons. One of defensive mechanisms is the PIWI-associated RNA (piRNA) pathway, which employs small RNAs for sequence-specific repression. The loss of the piRNA pathway in mice causes male sterility while females remain fertile. Unlike spermatogenic cells, mouse oocytes posses also RNA interference (RNAi), another small RNA pathway capable of retrotransposon suppression. To examine whether RNAi compensates the loss of the piRNA pathway, we produced a new RNAi pathway mutant DicerSOM and crossed it with a catalytically-dead mutant of Mili, an essential piRNA gene. Normal follicular and oocyte development in double mutants showed that RNAi does not suppress a strong ovarian piRNA knock-out phenotype. However, we observed redundant and non-redundant targeting of specific retrotransposon families illustrating stochasticity of recognition and targeting of invading retrotransposons. Intracisternal A Particle retrotransposon was mainly targeted by the piRNA pathway, MaLR and RLTR10 retrotransposons were targeted mainly by RNAi. Double mutants showed accumulations of LINE-1 retrotransposon transcripts. However, we did not find strong evidence for transcriptional activation and mobilization of retrotransposition competent LINE-1 elements suggesting that while both defense pathways are simultaneously expendable for ovarian oocyte development, yet another transcriptional silencing mechanism prevents mobilization of LINE-1 elements. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2020 Electronic address https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1008261
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