Macromolecular nanocrystal structural analysis with electron and X-rays: a comparative review

0521678 - FZÚ 2020 RIV CH eng J - Journal Article
Khakurel, Krishna - Angelov, Borislav - Andreasson, Jakob
Macromolecular nanocrystal structural analysis with electron and X-rays: a comparative review.
Molecules. Roč. 24, č. 19 (2019), s. 1-13, č. článku 3490. E-ISSN 1420-3049
R&D Projects: GA MŠMT LQ1606
Institutional support: RVO:68378271
Keywords : nanocrystallography * X-ray free-electron laser * electron diffraction * serial femtosecond crystallography * 3-dimensional fourier synthesis * protein-structure determination
OECD category: Fluids and plasma physics (including surface physics)
Impact factor: 3.267, year: 2019
Method of publishing: Open access

Crystallography has long been the unrivaled method that can provide the atomistic structural models of macromolecules, using either X-rays or electrons as probes. The methodology has gone through several revolutionary periods, driven by the development of new sources, detectors, and other instrumentation. Novel sources of both X-ray and electrons are constantly emerging. The increase in brightness of these sources, complemented by the advanced detection techniques, has relaxed the traditionally strict need for large, high quality, crystals. Recent reports suggest high-quality diffraction datasets from crystals as small as a few hundreds of nanometers can be routinely obtained. This has resulted in the genesis of a new field of macromolecular nanocrystal crystallography. Here we will make a brief comparative review of this growing field focusing on the use of X-rays and electrons sources.
Permanent Link: http://hdl.handle.net/11104/0306270