0521634 - FZÚ 2020 RIV US eng J - Journal Article
Bielecki, J. - Hantke, M.F. - Daurer, B.J. - Reddy, H.K.N. - Hasse, D. - Larsson, D.S.D. - Gunn, L.H. - Svenda, M. - Munke, A. - Sellberg, J.A. - Flueckiger, L. - Pietrini, A. - Nettelblad, C. - Lundholm, I. - Carlsson, G. - Okamoto, K. - Timneanu, N. - Westphal, D. - Kulyk, Olena - Higashiura, A. - van der Schot, G. - Loh, N.-T.D. - Wysong, T.E. - Bostedt, C. - Gorkhover, T. - Iwan, A. - Seibert, M.M. - Osipov, T. - Walter, P. - Hart, Ph. - Bucher, M. - Ulmer, A. - Ray, D. - Carini, G. - Ferguson, K.R. - Andersson, I. - Andreasson, Jakob - Hajdu, Janos - Maia, F.R.N.C.
Electrospray sample injection for single-particle imaging with x-ray lasers.
Science Advances. Roč. 5, č. 5 (2019), s. 1-9, č. článku eaav8801. ISSN 2375-2548. E-ISSN 2375-2548
R&D Projects: GA MŠMT LQ1606
Institutional support: RVO:68378271
Keywords : mass-spectrometry * crystallography * generation * mobility
OECD category: Fluids and plasma physics (including surface physics)
Impact factor: 13.117, year: 2019
Method of publishing: Open access

The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite high enough pulse intensity from currently available x-ray lasers and, as we demonstrate here, contamination of the aerosolized molecules by nonvolatile contaminants in the solution. The amount of contamination on the sample depends on the initial droplet size during aerosolization. Here, we show that, with our electrospray injector, we can decrease the size of aerosol droplets and demonstrate virtually contaminant-free sample delivery of organelles, small virions, and proteins. The results presented here, together with the increased performance of next-generation x-ray lasers, constitute an important stepping stone toward the ultimate goal of protein structure determination from imaging at room temperature and high temporal resolution.
Permanent Link: http://hdl.handle.net/11104/0306226