Non-thermal plasma, as a new physicochemical source, to induce redox imbalance and subsequent cell death in liver cancer cell lines

0521395 - FZÚ 2020 RIV CH eng J - Journal Article
Smolková, Barbora - Lunova, Mariia - Lynnyková, Anna - Uzhytchak, Mariia - Churpita, Olexandr - Jirsa, M. - Kubinová, Šárka - Lunov, Oleg - Dejneka, Alexandr
Non-thermal plasma, as a new physicochemical source, to induce redox imbalance and subsequent cell death in liver cancer cell lines.
Cellular Physiology and Biochemistry. Roč. 52, č. 1 (2019), s. 119-140. ISSN 1015-8987
R&D Projects: GA MPO(CZ) FV10081; GA MŠMT(CZ) EF16_019/0000760; GA AV ČR(CZ) Fellowship J. E. Purkyně
Grant - others:OP VVV - SOLID21(XE) CZ.02.1.01/0.0/0.0/16_019/0000760; AV ČR(CZ) Fellowship J. E. Purkyně
Program: Fellowship J. E. Purkyně
Institutional support: RVO:68378271
Keywords : oxidative stress * reactive oxygen species * apoptosis * non-thermal plasma * stress resistance
OECD category: Biophysics
Impact factor: 5.500, year: 2017
Method of publishing: Open access

In recent years, the emerged field of non-thermal plasma (NTP) has shown considerable promise in various biomedical applications, including cancer therapy. However, understanding the molecular mechanisms procuring cellular responses remains incomplete. Thus, the aim of this study was a rigorous biochemical analysis of interactions between NTP and liver cancer cells. We assessed the influence of NTP on three human liver cancer cell lines (Huh7, Alexander and HepG2). NTP treatment resulted in higher anti-proliferative effect against Alexander and Huh7 relative to HepG2. Our data clearly showed that the NTP-mediated alternation of mitochondrial membrane potential and dynamics led to ROS-mediated apoptosis in Huh7 and Alexander cells. Interestingly, plasma treatment resulted in p53 down-regulation in Huh7 cells. High levels of Bcl-2 protein expression in HepG2 resulted in their resistance in response to oxidative stress- mediated by plasma.
Permanent Link: http://hdl.handle.net/11104/0306026