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Method of detection of analyte active forms and determination of the ability of substances to bind into analyte active sites
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SYSNO ASEP 0520784 Document Type P - Patent R&D Document Type Patent or other outcome protected by special legislation Title Method of detection of analyte active forms and determination of the ability of substances to bind into analyte active sites Author(s) Navrátil, Václav (UOCHB-X) RID, ORCID
Šácha, Pavel (UOCHB-X) RID, ORCID
Schimer, Jiří (UOCHB-X) RID, ORCID
Konvalinka, Jan (UOCHB-X) RID, ORCID
Majer, Pavel (UOCHB-X)Year of issue 2019 Possible third party use of the result A - Pro využití výsledku jiným subjektem je vždy nutné nabytí licence Royalty requested A - Poskytovatel licence požaduje licenční poplatek Patent no. or utility model no. or industrial design no. CA2957286 Date of the patent acceptance 06.08.2019 Name of the patent owner Ústav organické chemie a biochemie AV ČR, v. v. i Code of the issuer name CA001 - Canadian Intellectual Property Office (CIPO) Gatineau, Québec Current use B - Patent je využíván na základě uzavřené licenční smlouvy mezi vlastníkem a uživatelem Language eng - English Keywords DIANA ; inhibitor ; analyt Subject RIV CE - Biochemistry OECD category Biochemical research methods R&D Projects LO1302 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) NV15-31379A GA MZd - Ministry of Health (MZ) Institutional support UOCHB-X - RVO:61388963 Annotation The invention provides a method for detection of active form of analytes in a sample and/or for determination of ability of tested substances to bind to the active site of these analytes, comprising the following steps: a) analyte or group of analytes from the sample is immobilized on the surface of a solid carrier either by non-specific non-covalent adsorption or by covalent binding of surface functional groups of the analyte and corresponding functional groups of the solid carrier, or preferably via a binding molecule which is bound to the surface of the solid carrier before immobilization of the analyte or group of analytes and is capable of selectively binding the analyte or group of analytes contained in the sample during incubation of the solid carrier with the sample, b) analyte or group of analytes is incubated with a detection probe which binds selectively to the analyte or group of analytes via a compound for selective binding to the analyte active site, whereas the probe consists of a low molecular compound for selective binding to the analyte active site, an oligonucleotide tag, optionally with a covalently attached fluorophore, biotin or a chemical group, and a chemical linker covalently linking the compound for selective binding to the analyte active site and the oligonucleotide tag, c) then the solid carrier is washed to remove unbound detection probe, and subsequently, the amount of bound detection probe is determined, whereas this amount is directly proportional to the amount of the analyte or group of analytes in the sample. The described method has broad application in medicine. Given the exceptional sensitivity of only a few dozen molecules, it provides the ability to determine the protein markers in blood in a concentration yet undetectable. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2020 Electronic address https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=CA&NR=2957286C&KC=C&FT=D&ND=4&date=20190806&DB=&locale=en_EP#
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