Number of the records: 1
NMR metabolomics reveals effects of Cryptosporidium infections on host cell metabolome
- 1.
SYSNO ASEP 0520226 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title NMR metabolomics reveals effects of Cryptosporidium infections on host cell metabolome Author(s) Miller, C.N. (GB)
Panagos, C.G. (GB)
Mosedale, W.R.T. (GB)
Kváč, Martin (BC-A) RID, RID, ORCID
Howard, M.J. (GB)
Tsaousis, A.D. (CA)Number of authors 6 Article number 13 Source Title Gut Pathogens. - : BioMed Central - ISSN 1757-4749
Roč. 11, APR 3 2019 (2019)Number of pages 12 s. Publication form Online - E Language eng - English Country GB - United Kingdom Keywords acquired-immunodeficiency-syndrome ; energy-metabolism ; oxidative stress ; parvum ; taurine ; culture ; burden ; epidemiology ; apicomplexan ; excystation ; Cryptosporidiosis ; nmr ; Metabolomics ; colo-680n ; Taurine Subject RIV GJ - Animal Vermins ; Diseases, Veterinary Medicine OECD category Veterinary science R&D Projects GA15-01090S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 000463842900001 EID SCOPUS 85063929769 DOI 10.1186/s13099-019-0293-x Annotation BackgroundCryptosporidium is an important gut microbe whose contributions towards infant and immunocompromise patient mortality rates are steadily increasing. Over the last decade, we have seen the development of various tools and methods for studying Cryptosporidium infection and its interactions with their hosts. One area that is sorely overlooked is the effect infection has on host metabolic processes.ResultsUsing a H-1 nuclear magnetic resonance approach to metabolomics, we have explored the nature of the mouse gut metabolome as well as providing the first insight into the metabolome of an infected cell line. Statistical analysis and predictive modelling demonstrated new understandings of the effects of a Cryptosporidium infection, while verifying the presence of known metabolic changes. Of note is the potential contribution of host derived taurine to the diarrhoeal aspects of the disease previously attributed to a solely parasite-based alteration of the gut environment, in addition to other metabolites involved with host cell catabolism.ConclusionThis approach will spearhead our understanding of the Cryptosporidium-host metabolic exchange and provide novel targets for tackling this deadly parasite. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2020 Electronic address https://gutpathogens.biomedcentral.com/track/pdf/10.1186/s13099-019-0293-x
Number of the records: 1