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Development of breeding methods for increased lipids accumulation by marine microalgae using fluorescence-activated cell sorting
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SYSNO ASEP 0518558 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Development of breeding methods for increased lipids accumulation by marine microalgae using fluorescence-activated cell sorting Author(s) Búzová, Diana (UEK-B) SAI, ORCID, RID
Literáková, Petra (UEK-B) ORCID, RID, SAI
Fedorko, Jan (UEK-B) SAI, ORCID
Kaštánek, P. (CZ)
Červený, Jan (UEK-B) RID, ORCID, SAISource Title Book of abstract: 9th European Symposium on Plant Lipids. - Marseille, 2019 / Beisson Fred ; Li-Beisson Yonghua
S. 52-53Number of pages 1 s. Action 9th European Symposium on Plant Lipids Event date 07.07.2019 - 10.07.2019 VEvent location Marseille Country FR - France Event type EUR Language eng - English Country FR - France Keywords breeding methods ; lipids accumulation ; fluorescence-activated cell sorting ; microalgae R&D Projects TN01000048 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) LO1415 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015055 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEK-B - RVO:86652079 Annotation Development of breeding methods for increased lipids accumulation by marine microalgae using fluorescence-activated cell sorting. The marine microorganism belonging to the family Thraustochytriidae are group of non-photosynthetic marine protists. These unicellular, heterotrophic eukaryotes are commonly found in seawater and sediments, with the highest abundance in nutrient-rich areas, such as mangrove forests. Thraustochytrids are reported to be efficient producers of polyunsaturated fatty acids (PUFA) including the n-3 series, such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). These organisms are considered as one of the potential alternative sources of PUFA for commercial and industrial exploitation. We developed advanced cultivation strategy and breeding method, and applied them on one particular Thraustochytrid strain Schizochytrium AN4 for optimization of lipids accumulation. The breeding method aims for selection of the hyper producing subpopulation using high-throughput fluorescence-activated cell sorting (FACS) in combination with imaging flow cytometry (IFC) and multiparametric discrimination the cells with both high lipid content and metabolic activity. The method was tested for negative influence on post-sorting cell viability possibly caused by the sorting technique procedure (e.g. high pressure, laser beam exposure, electro-magnetic field, toxic effects of both fluorescent marker and fluidic system medium composition) with no effect compared to non-sorted control sample. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2020
Number of the records: 1