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A New Approach for the Diagnosis of Myelodysplastic Syndrome Subtypes Based on Protein Interaction Analysis
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SYSNO ASEP 0518336 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A New Approach for the Diagnosis of Myelodysplastic Syndrome Subtypes Based on Protein Interaction Analysis Author(s) Chrastinová, L. (CZ)
Pastva, O. (CZ)
Bocková, Markéta (URE-Y)
Lynn, Nicholas Scott (URE-Y) RID
Šácha, Pavel (UOCHB-X) RID, ORCID
Hubálek, Martin (UOCHB-X) RID, ORCID
Suttnar, J. (CZ)
Kotlín, R. (CZ)
Štikarová, J. (CZ)
Hlaváčková, A. (CZ)
Pimková, K. (CZ)
Čermák, J. (CZ)
Homola, Jiří (URE-Y) RID
Dyr, J. E. (CZ)Number of authors 14 Article number 12647 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 9, č. 1 (2019)Number of pages 10 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords Biosensor ; MicroRNAs ; Leukemia, Myeloid, Acute Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry Subject RIV - cooperation Institute of Organic Chemistry and Biochemistry - Biochemistry R&D Projects GBP205/12/G118 GA ČR - Czech Science Foundation (CSF) GA19-02739S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support URE-Y - RVO:67985882 ; UOCHB-X - RVO:61388963 UT WOS 000483697800029 EID SCOPUS 85071766635 DOI 10.1038/s41598-019-49084-2 Annotation Myelodysplastic syndromes (MDS) are a heterogeneous group of hematological malignancies with a high risk of transformation to acute myeloid leukemia (AML). MDS are associated with posttranslational modifications of proteins and variations in the protein expression levels. In this work, we present a novel interactomic diagnostic method based on both protein array and surface plasmon resonance biosensor technology, which enables monitoring of protein-protein interactions in a label-free manner. In contrast to conventional methods based on the detection of individual biomarkers, our presented method relies on measuring interactions between arrays of selected proteins and patient plasma. We apply this method to plasma samples obtained from MDS and AML patients, as well as healthy donors, and demonstrate that even a small protein array comprising six selected proteins allows the method to discriminate among different MDS subtypes and healthy donors. Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2020 Electronic address https://www.nature.com/articles/s41598-019-49084-2
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