T cells in swine completely rearrange immunoglobulin heavy chain genes

0517717 - MBÚ 2020 RIV GB eng J - Journal Article
Šinkorová, Jana - Štěpánová, Kateřina - Butler, J. E. - Šinkora, Marek
T cells in swine completely rearrange immunoglobulin heavy chain genes.
Developmental and Comparative Immunology. Roč. 99, OCT 2019 (2019), č. článku UNSP 103396. ISSN 0145-305X. E-ISSN 1879-0089
R&D Projects: GA ČR(CZ) GA16-09296S
Institutional support: RVO:61388971
Keywords : Porcine immune system * B cell receptors * Gene rearrangement
OECD category: Zoology
Impact factor: 3.192, year: 2019
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0145305X19301491?via%3Dihub

Porcine thymus contains three independent populations of cells that have rearranged immunoglobulin heavy chain VDJ(H) genes. The first population can be found exclusively in medulla and it consists of existing mature B cells and plasma cells. The second consists of developing B cells characterized by the presence of selected VDJ(H )rearrangement, similar to B cell lymphogenesis in the bone marrow. The third population is entirely unaffected by selection mechanism for productive VDJ(H )rearrangement and represents T lineage cells that rearrange immunoglobulin genes. Transcription of unselected VDJ(H) repertoire is not allowed in T cells. Sequence analysis of unselected VDJ(H) repertoire from T cells also revealed important consequences for B cell lymphogenesis and selection of B cell repertoire. As far as we know, this is the first evidence that some species completely rearrange VDJ(H )genes in T cells. Our results also support the finding that B cells actively develop in the thymus.
Permanent Link: http://hdl.handle.net/11104/0303010