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Dual Mode of the Saponin Aescin in Plant Protection: Antifungal Agent and Plant Defense Elicitor
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SYSNO ASEP 0512060 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Dual Mode of the Saponin Aescin in Plant Protection: Antifungal Agent and Plant Defense Elicitor Author(s) Trdá, Lucie (UEB-Q) ORCID
Janda, Martin (UEB-Q) RID, ORCID
Macková, Denisa (UEB-Q)
Pospíchalová, Romana (UEB-Q)
Dobrev, Petre (UEB-Q) RID, ORCID
Burketová, Lenka (UEB-Q) RID, ORCID
Matušinský, P. (CZ)Article number 1448 Source Title Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 10, Nov 28 (2019)Number of pages 14 s. Language eng - English Country CH - Switzerland Keywords Brassica napus ; Leptosphaeria maculans ; salicylic acid Subject RIV EI - Biotechnology ; Bionics OECD category Biochemical research methods R&D Projects QK1910197 GA MZe - Ministry of Agriculture (MZe) TG03010009 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000505261700001 EID SCOPUS 85076921864 DOI 10.3389/fpls.2019.01448 Annotation Being natural plant antimicrobials, saponins have potential for use as biopesticides. Nevertheless, their activity in plant–pathogen interaction is poorly understood. We performed a comparative study of saponins' antifungal activities on important crop pathogens based on their effective dose (EC50) values. Among those saponins tested, aescin showed itself to be the strongest antifungal agent. The antifungal effect of aescin could be reversed by ergosterol, thus suggesting that aescin interferes with fungal sterols. We tested the effect of aescin on plant–pathogen interaction in two different pathosystems: Brassica napus versus (fungus) Leptosphaeria maculans and Arabidopsis thaliana versus (bacterium) Pseudomonas syringae pv tomato DC3000 (Pst DC3000). We analyzed resistance assays, defense gene transcription, phytohormonal production, and reactive oxygen species production. Aescin activated B. napus defense through induction of the salicylic acid pathway and oxidative burst. This defense response led finally to highly efficient plant protection against L. maculans that was comparable to the effect of fungicides. Aescin also inhibited colonization of A. thaliana by Pst DC3000, the effect being based on active elicitation of salicylic acid (SA)-dependent immune mechanisms and without any direct antibacterial effect detected. Therefore, this study brings the first report on the ability of saponins to trigger plant immune responses. Taken together, aescin in addition to its antifungal properties activates plant immunity in two different plant species and provides SA-dependent resistance against both fungal and bacterial pathogens. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2020 Electronic address http://doi.org/10.3389/fpls.2019.01448
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