Comparative Electrochemical and Spectroscopic Studies of I-motif-forming DNA Nonamers

0511674 - BFÚ 2020 RIV DE eng J - Journal Article
Trnková, L. - Třísková, I. - Vorlíčková, Michaela - Kejnovská, Iva - Dvořáková, Zuzana - Pivoňková, Hana - Fiala, R.
Comparative Electrochemical and Spectroscopic Studies of I-motif-forming DNA Nonamers.
Electroanalysis. Roč. 31, č. 10 (2019), s. 2081-2093. ISSN 1040-0397. E-ISSN 1521-4109
R&D Projects: GA ČR(CZ) GA19-17063S
Institutional support: RVO:68081707
Keywords : elimination voltammetry * circular-dichroism * cytosine signals * guanine residues * telomeric dna
OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Impact factor: 2.544, year: 2019
Method of publishing: Limited access
https://onlinelibrary.wiley.com/doi/abs/10.1002/elan.201900323

The paper shows the structural diversity of cytosine (C)-rich oligodeoxynucleotides (ODNs) arising from their detail nucleotide sequence and experimental conditions. In slightly acidic solutions, the ODN nonamers with different adenine (A) and cytosine (C) sequences can adopt non-canonical structures involving protonated bases. A distinct secondary structure formed in (C)-rich sequences, called i-motif (iM), consists of hemiprotonated and intercalated cytosine base pairs (C.C+). Folding and unfolding of particular structures in solutions were monitored by H-1 NMR and CD spectroscopies and native polyacrylamide gel electrophoresis (PAGE), which are capable to determine their structural characteristics. Effects of sequences and their proclivity to formation of the iM on electrochemical behaviour of the ODN nonamers were studied by electrochemical methods. The LSV signals of A and C obtained from the reductive dissolution of ODN adsorption layers on a hanging mercury drop electrode were processed by elimination voltammetry with linear scan (EVLS), which revealed complex effects of the nonamer properties (namely their primary and secondary structure confirmed in solution) on their adsorption and reduction activity.
Permanent Link: http://hdl.handle.net/11104/0301904