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Interaction pathways and structure-chemical transformations of alginate gels in physiological environments
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SYSNO ASEP 0510805 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Interaction pathways and structure-chemical transformations of alginate gels in physiological environments Author(s) Urbanová, Martina (UMCH-V) RID, ORCID
Pavelková, M. (CZ)
Czernek, Jiří (UMCH-V) RID
Kubová, K. (CZ)
Vysloužil, J. (CZ)
Pechová, A. (CZ)
Molinková, D. (CZ)
Vysloužil, J. (CZ)
Vetchý, D. (CZ)
Brus, Jiří (UMCH-V) RID, ORCIDSource Title Biomacromolecules. - : American Chemical Society - ISSN 1525-7797
Roč. 20, č. 11 (2019), s. 4158-4170Number of pages 13 s. Language eng - English Country US - United States Keywords alginate gels ; molecular-level interactions ; physiological conditions Subject RIV CD - Macromolecular Chemistry OECD category Polymer science R&D Projects LO1507 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LTAUSA18011 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support UMCH-V - RVO:61389013 UT WOS 000496343800012 EID SCOPUS 85074441891 DOI https://doi.org/10.1021/acs.biomac.9b01052 Annotation The remarkably diverse affinity of alginate (ALG) macromolecules for polyvalent metal ions makes cross-linked alginate gels an outstanding biomaterial. Surprisingly, however, very little is known about their interactions and structural transformations in physiological environments. To bridge this gap, we prepared a set of ALG gels cross-linked by various ions and monitored their structural changes at different media simulating gastric and intestinal fluids and cellular environments. For these studies, we used multinuclear solid-state NMR (ss-NMR) spectroscopy, which revealed a range of competitive ion-exchange and interconversion reactions, the rate of which strongly depended on the nature of the cross-linking metal ions. Depending on the environment, ALG chains adopted different forms, such as acidic (hydro)gels stabilized by strong hydrogen bonds, and/or weakly cross-linked Na/H-gels. Simultaneously, the exchanged polyvalent ions extensively interacted with the environment even forming in some cases insoluble phosphate microdomains directly deposited in the ALG bead matrix. The extent of the transformations and incorporation of secondary phases into the alginate beads followed the size and electronegativity of the cross-linking ions. Overall, the applied combination of various macroscopic and biological tests with multinuclear ss-NMR revealed a complex pathway of alginate beads transformations in physiological environments. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2020 Electronic address https://pubs.acs.org/doi/10.1021/acs.biomac.9b01052
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