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Non-Natural Nucleic Acids: Methods and Protocols
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SYSNO ASEP 0509903 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Synthesis of Base-Modified dNTPs Through Cross-Coupling Reactions and Their Polymerase Incorporation to DNA Author(s) Ménová, Petra (UOCHB-X) RID
Cahová, Hana (UOCHB-X) RID, ORCID
Vrábel, Milan (UOCHB-X) RID, ORCID
Hocek, Michal (UOCHB-X) RID, ORCIDSource Title Non-Natural Nucleic Acids: Methods and Protocols. - New York : Humana Press, 2019 / Shank N. - ISSN 1064-3745 - ISBN 978-1-4939-9215-7 Pages s. 39-57 Number of pages 19 s. Number of pages 315 Publication form Print - P Language eng - English Country US - United States Keywords nucleotides ; nucleoside triphosphates ; DNA polymerases ; enzymatic synthesis ; modified DNA Subject RIV CC - Organic Chemistry OECD category Organic chemistry R&D Projects GA18-03305S GA ČR - Czech Science Foundation (CSF) EF16_019/0000729 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UOCHB-X - RVO:61388963 UT WOS 000487217700005 EID SCOPUS 85065338413 DOI 10.1007/978-1-4939-9216-4_4 Annotation Synthesis of base-modified dNTPs through the Suzuki or Sonogashira cross-coupling reactions of halogenated dNTPs with boronic acids or alkynes is reported, as well as the use of these modified dNTPs in polymerase incorporations to oligonucleotides or DNA by primer extension or PCR. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2020
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