Ivermectin biotransformation and impact on transcriptome in Arabidopsis thaliana

Syslová, Eliška; Landa, Přemysl; Navrátilová, M.; Stuchlíková Raisová, L.; Matoušková, P.; Skálová, L.; Szotáková, B.; Vaněk, Tomáš; Podlipná, Radka

doi:https://dx.doi.org/10.1016/j.chemosphere.2019.06.102

Number of the records: 1

Ivermectin biotransformation and impact on transcriptome in Arabidopsis thaliana

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SYSNO ASEP	0509662
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Ivermectin biotransformation and impact on transcriptome in Arabidopsis thaliana
Author(s)	Syslová, Eliška (UEB-Q) Landa, Přemysl (UEB-Q)_{RID, ORCID} Navrátilová, M. (CZ) Stuchlíková Raisová, L. (CZ) Matoušková, P. (CZ) Skálová, L. (CZ) Szotáková, B. (CZ) Vaněk, Tomáš (UEB-Q)_{RID, ORCID} Podlipná, Radka (UEB-Q)_{RID, ORCID}
Number of authors	9
Source Title	Chemosphere. - : Elsevier - ISSN 0045-6535 Roč. 234, NOV (2019), s. 528-535
Number of pages	8 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	Arabidopsis thaliana ; Ivermectin ; Metabolites ; Transcriptomic response ; Veterinary drug
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Pharmacology and pharmacy
R&D Projects	GA18-08452S GA ČR - Czech Science Foundation (CSF)
	GA18-07724S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Open access
Institutional support	UEB-Q - RVO:61389030
UT WOS	000488136200058
EID SCOPUS	85067502101
DOI	10.1016/j.chemosphere.2019.06.102
Annotation	Veterinary drugs enter the environment in many ways and may affect non-target organisms, including plants. The present project was focused on the biotransformation of ivermectin (IVM), one of the mostly used anthelmintics, in the model plant Arabidopsis thaliana. Our results certified the ability of plants to uptake IVM by roots and translocate it to the aboveground parts. Using UHPLC-MS/MS, six metabolites in roots and only the parent drug in rosettes were found after 24- and 72-h incubation of A. thaliana with IVM. The metabolites were formed only via hydroxylation and demethylation, with no IVM conjugates detected. Although IVM did not induce changes in the activity of antioxidant enzymes in A. thaliana rosettes, the expression of genes was significantly affected. Surprisingly, a higher number of transcripts, 300 and 438, respectively, was dysregulated in the rosettes than in roots. The significantly affected genes play role in response to salt, osmotic and water deprivation stress, in response to pathogens and in ion homeostasis. We hypothesize that the above described changes in gene transcription in A. thaliana resulted from disrupted ionic homeostasis caused by certain ionophore properties of IVM. Our results underlined the negative impact of IVM presence in the environment.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2020
Electronic address	http://dx.doi.org/10.1016/j.chemosphere.2019.06.102

Number of the records: 1