Interplay between phosphoinositides and actin cytoskeleton in the regulation of immunity related responses in Arabidopsis thaliana seedlings

0509290 - ÚEB 2020 RIV NL eng J - Journal Article
Kalachova, Tetiana - Leontovyčová, Hana - Iakovenko, Oksana - Pospíchalová, Romana - Maršík, P. - Klouček, P. - Janda, Martin - Valentová, O. - Kocourková, Daniela - Martinec, Jan - Burketová, Lenka - Ruelland, E.
Interplay between phosphoinositides and actin cytoskeleton in the regulation of immunity related responses in Arabidopsis thaliana seedlings.
Environmental and Experimental Botany. Roč. 167, NOV (2019), č. článku 103867. ISSN 0098-8472. E-ISSN 1873-7307
R&D Projects: GA ČR GA17-05151S; GA MŠMT(CZ) EF16_019/0000738
Grant - others:OPPK(XE) CZ.2.16/3.1.00/21519
Institutional support: RVO:61389030
Keywords : Actin * Callose * Latrunculin B * phosphatidylinositol-4-kinase * Salicylic acid
OECD category: Cell biology
Impact factor: 4.027, year: 2019
Method of publishing: Open access
http://dx.doi.org/10.1016/j.envexpbot.2019.103867

Actin cytoskeleton is indispensable for plant cell integrity. Besides, increasing evidences illustrate its crucial role in plant responses to environment, including defence against pathogens. Recently, we have demonstrated that pre-treatment with actin disrupting drugs latrunculin B (latB) and cytochalasin E can enhance plant resistance against bacterial and fungal pathogens via activation of salicylic acid (SA) pathway. Here, we show that actin depolymerization in Arabidopsis thaliana seedlings not only triggers SA biosynthesis by ICS1, but also induces callose deposition via callose synthase PMR4. This effect is SA-independent since still present in mutants that do not accumulate SA. LatB also triggers the expression of several defence related genes. We could distinguish genes, induced in a SA-dependent manner (PR1, WRKY38, ICS1) and those that are SA-independent (PR2, PAD4, BAP1). As actin cytoskeleton is tightly connected with membrane trafficking, we assayed the effect of latB on mutant plants invalidated in phosphatidylinositol 4-kinase beta1 and beta2 (PI4Kβ1β2). Deficiency in PI4Kβ1β2 enhanced latB-triggered actin filaments depolymerisation. Yet, it did not lead to a stronger callose deposition or SA biosynthesis in response to latB. Surprisingly, introduction of NahG construct or pad4 mutation in pi4kß1ß2 background had much lower effect on SA induction and SA-dependent gene expression changes than it has in wild type. We can thus conclude that actin disruption itself triggers immune-like responses: there is an induction of SA via PAD4 coupled to ICS1, it leads to the induction of PR1 and WRKY38, and this requires a functional PI4Kβ1β2 to be properly regulated. However, an alternative, SA-independent pathway also exists that leads to the enhanced expression of PR2 and to callose accumulation.
Permanent Link: http://hdl.handle.net/11104/0300111