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Deregulated Phosphorylation of CENH3 at Ser65 Affects the Development of Floral Meristems in Arabidopsis thaliana
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SYSNO ASEP 0507848 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Deregulated Phosphorylation of CENH3 at Ser65 Affects the Development of Floral Meristems in Arabidopsis thaliana Author(s) Demidov, D. (DE)
Heckmann, S. (US)
Weiss, O. (DE)
Rutten, T. (DE)
Tomaštíková, Eva (UEB-Q) RID, ORCID
Kuhlmann, M. (DE)
Scholl, P. (AT)
Municio, C. M. (DE)
Lermontova, I. (DE)
Houben, A. (DE)Number of authors 10 Article number 928 Source Title Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 10, JUL 25 (2019)Number of pages 11 s. Language eng - English Country CH - Switzerland Keywords aurora-b kinase ; histone h3 ; chromosome segregation ; centromeric chromatin ; protein ; deposition ; identification ; expression ; division ; dynamics ; cenh3 ; phosphorylation ; Aurora kinase ; floral meristem ; Arabidopsis Subject RIV ED - Physiology OECD category Biochemistry and molecular biology R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000477060000001 EID SCOPUS 85072196084 DOI 10.3389/fpls.2019.00928 Annotation Several histone variants are posttranslationally phosphorylated. Little is known about phosphorylation of the centromere-specific histone 3 (CENH3) variant in plants. We show that CENH3 of Arabidopsis thaliana is phosphorylated in vitro by Aurora3, predominantly at serine 65. Interaction of Aurora3 and CENH3 was found by immunoprecipitation (IP) in A. thaliana and by bimolecular fluorescence complementation. Western blotting with an anti-CENH3 pS65 antibody showed that CENH3 pS65 is more abundant in flower buds than elsewhere in the plant. Substitution of serine 65 by either alanine or aspartic acid resulted in a range of phenotypic abnormalities, especially in reproductive tissues. We conclude that Aurora3 phosphorylates CENH3 at S65 and that this post-translational modification is required for the proper development of the floral meristem. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2020 Electronic address http://dx.doi.org/10.3389/fpls.2019.00928
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