HIF-1 alpha is required for development of the sympathetic nervous system

Bohuslavová, Romana; Čerychová, Radka; Papoušek, František; Olejníčková, Veronika; Bartoš, M.; Goerlach, A.; Kolář, František; Sedmera, David; Semenza, G.L.; Pavlínková, Gabriela

doi:https://dx.doi.org/10.1073/pnas.1903510116

Number of the records: 1

HIF-1 alpha is required for development of the sympathetic nervous system

1.

SYSNO ASEP	0507766
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	HIF-1 alpha is required for development of the sympathetic nervous system
Author(s)	Bohuslavová, Romana (BTO-N)_RID Čerychová, Radka (BTO-N) Papoušek, František (FGU-C) Olejníčková, Veronika (FGU-C)_{RID, ORCID, SAI} Bartoš, M. (CZ) Goerlach, A. (DE) Kolář, František (FGU-C)_{RID, ORCID, SAI} Sedmera, David (FGU-C)_{RID, ORCID, SAI} Semenza, G.L. (US) Pavlínková, Gabriela (BTO-N)_{RID, ORCID}
Number of authors	10
Source Title	Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424 Roč. 116, č. 27 (2019), s. 13414-13423
Number of pages	10 s.
Language	eng - English
Country	US - United States
Keywords	sympathetic neurons ; tyrosine hydroxylase ; cardiac innervation
Subject RIV	FH - Neurology
OECD category	Cardiac and Cardiovascular systems
Subject RIV - cooperation	Institute of Physiology - Cardiovascular Diseases incl. Cardiotharic Surgery
R&D Projects	GA17-04719S GA ČR - Czech Science Foundation (CSF)
	GA19-07378S GA ČR - Czech Science Foundation (CSF)
	LTC17023 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Research Infrastructure	Czech-BioImaging - 90062 - Ústav molekulární genetiky AV ČR, v. v. i.
Method of publishing	Open access
Institutional support	BTO-N - RVO:86652036 ; FGU-C - RVO:67985823
UT WOS	000473427900043
EID SCOPUS	85068259608
DOI	10.1073/pnas.1903510116
Annotation	The molecular mechanisms regulating sympathetic innervation of the heart during embryogenesis and its importance for cardiac development and function remain to be fully elucidated. We generated mice in which conditional knockout (CKO) of the Hif1a gene encoding the transcription factor hypoxia-inducible factor 1 alpha (HIF-1 alpha) is mediated by an Islet1-Cre transgene expressed in the cardiac outflow tract, right ventricle and atrium, pharyngeal mesoderm, peripheral neurons, and hindlimbs. These Hif1aCKO mice demonstrate significantly decreased perinatal survival and impaired left ventricular function. The absence of HIF-1 alpha impaired the survival and proliferation of preganglionic and postganglionic neurons of the sympathetic system, respectively. These defects resulted in hypoplasia of the sympathetic ganglion chain and decreased sympathetic innervation of the Hif1aCKO heart, which was associated with decreased cardiac contractility. The number of chromaffin cells in the adrenal medulla was also decreased, indicating a broad dependence on HIF-1 alpha for development of the sympathetic nervous system.
Workplace	Institute of Biotechnology
Contact	Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700
Year of Publishing	2020
Electronic address	https://doi.org/10.1073/pnas.1903510116

Number of the records: 1