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WHOLE-GENOME EXPRESSION ANALYSIS IN THP-1 MACROPHAGE-LIKE CELLS EXPOSED TO DIVERSE NANOMATERIALS
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SYSNO ASEP 0507359 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title WHOLE-GENOME EXPRESSION ANALYSIS IN THP-1 MACROPHAGE-LIKE CELLS EXPOSED TO DIVERSE NANOMATERIALS Author(s) Brzicová, Táňa (UEM-P)
Líbalová, Helena (UEM-P) RID
Vrbová, Kristýna (UEM-P)
Sikorová, Jitka (UEM-P)
Philimonenko, Vlada (UMG-J)
Kléma, J. (CZ)
Topinka, Jan (UEM-P) RID, ORCID
Rössner ml., Pavel (UEM-P) RID, ORCIDSource Title Nanocon 2017 : conference proceedings : 9th International Conference on Nanomaterials - Research & Application. - Ostrava : Tanger Ltd., 2018 - ISBN 9788087294819 Pages s. 679-684 Number of pages 6 s. Publication form Print - P Action International Conference on Nanomaterials - Research and Application (NANOCON) /9./ Event date 18.10.2017 - 20.10.2017 VEvent location Brno Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords nanomaterials ; toxicity ; THP-1 macrophages ; gene expression profiling Subject RIV EB - Genetics ; Molecular Biology OECD category Toxicology Subject RIV - cooperation Institute of Molecular Genetics - Genetics ; Molecular Biology R&D Projects LM2015073 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEM-P - RVO:68378041 ; UMG-J - RVO:68378050 UT WOS 000452823300112 EID SCOPUS 85051851925 Annotation From the perspective of the immune system, nanomaterials (NMs) represent invading agents. Macrophages are immune cells residing in all organs and tissues as the first line of defense. Interactions of macrophages with NMs can determine the fate of NMs as well as their potential toxic effects. In the present study, we compared toxicity of four different types of NMs [NM-100 (TiO2, 110 nm), NM-110 (ZnO, 20 nm), NM-200 (SiO2, 150 nm) and NM-300K (Ag, 20 nm)], towards THP-1 macrophage-like cells. Cells were incubated with non-cytotoxic concentrations (1-25 mu g/ml) of NMs for 24 hours and microarray technology was used to analyze changes in whole-genome expression. Gene expression profiling revealed a substantially different molecular response following exposure to diverse NMs. While NM-100 did not exert any significant effect on gene expression profile, all other NMs triggered a pro-inflammatory response characterized by an activation of the NF-kappa B transcription factor and induced expression of numerous chemokines and cytokines. NM-110 and NM-300K further modulated processes such as DNA damage response, oxidative and replication stress as well as cell cycle progression and proteasome function. We suppose that genotoxicity of ZnO and Ag NMs leading to DNA damage and alternatively to apoptosis in THP-1 macrophages is probably caused by the extensive intracellular dissolution of these NPs, as confirmed by TEM imaging. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2020
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